| 鸡新城疫卵黄抗体IgY的分离提纯研究 |
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| 引用本文： | 王洪新,刘学贤,穆海波,吕晓娟.鸡新城疫卵黄抗体IgY的分离提纯研究[J].中国家禽,2003,25(19):11-14. |
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| 作者姓名： | 王洪新 刘学贤 穆海波 吕晓娟 |
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| 作者单位： | 1. 江南大学食品学院,无锡,214036
2. 江苏省家禽科学研究所,扬州,225003 |
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| 摘 要： | 本文对母鸡经鸡新城疫疫苗免疫接种后产生并富集在卵黄中的抗体IgY的提取纯化工艺条件进行了研究与优化，确定了去脂、提取抗体最佳工艺为：卵黄液用0．05mol／L,pH5.0的乙酸一乙酸钠缓冲液1：9倍稀释，搅拌15min，4℃静置10小时，抗体回收率为90％，去脂率为99％；该提取液用40％饱和度的硫酸铵盐析，得到的盐析液抗体回收率为82％，纯度达到69％。盐析液经截留分子量为100KD的有机陶瓷膜超滤后，截留液抗体回收率达到92％。纯度为85％。
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| 关 键 词： | 鸡 新城疫 卵黄抗体IgY 分离 提纯 |
Study on the Extraction and Separation of Yolk Antibody IgY against Newcastle Disease |
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| Wang Hongxin ,Liu Xuexian ,Mu Haibo ,Lu Xiaojuan.Study on the Extraction and Separation of Yolk Antibody IgY against Newcastle Disease[J].China Poultry,2003,25(19):11-14. |
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| Authors: | Wang Hongxin Liu Xuexian Mu Haibo Lu Xiaojuan |
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| Institution: | Wang Hongxin 1,Liu Xuexian 2,Mu Haibo 1,Lu Xiaojuan 2 |
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| Abstract: | The optimal extraction conditions of yolk antibody IgY against Newcastle Disease(ND)were as following:egg yolk was diluted9times by acetic acid buffer(0.05mol/L,pH5.0),stirred15min,extracted10h at40C.The recovery of the IgY was90%and removing efficiency of fat was99%in this stage.The extracting solution was further precipitated with40%ammonium sulfate and the recovery of the IgY was82%.The precipitate was resolved and ultrafiltered through the organic ceramic membrane which molecular weight cut-off(MWCO)was100KD,and the recovery of the IgY in the Cutting-off solution was92%and the purity of the IgY was85%. |
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| Keywords: | antibody IgY against chicken Newcastle Disease extraction separation |
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