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| 蛋鸡血管瘤型J亚群禽白血病病毒的分离与鉴定 | |
| 引用本文： | 侯新华,申茂欣,鹿欣伦,楚电峰,刘东,孙健,左青山,杜元钊.蛋鸡血管瘤型J亚群禽白血病病毒的分离与鉴定[J].中国家禽,2011,33(3). |
| 作者姓名： | 侯新华 申茂欣 鹿欣伦 楚电峰 刘东 孙健 左青山 杜元钊 |
| 作者单位： | 1. 扬州大学兽医学院农业部畜禽传染病学重点开放实验室;江苏扬州,225009;青岛易邦生物工程有限公司,山东青岛266032 2. 扬州大学兽医学院农业部畜禽传染病学重点开放实验室,江苏扬州,225009 3. 青岛易邦生物工程有限公司,山东青岛,266032 |
| 基金项目： | "十一五"国家科技支撑计划项目 |
| 摘 要： | 通过临床观察、剖检、接种DF-1细胞、间接免疫荧光试验和PCR等方法,从安徽父母代种鸡场、青岛平度和沙子口某商品蛋鸡场分离到3株血管瘤型J亚群禽白血病病毒(ALV-J),分别命名为AH-101、PD-101、SZK-101.采集病鸡的血液、肝脏和脾脏组织,接种DF-1细胞分离病毒,提取DNA,用P11/PJ2 ALV-J引物进行PCR扩增结果为阳性,IFA检测为阳性.用J3/J4引物扩增gp85并测序,序列分析发现,3株毒株与国内外各ALV-J的相应核苷酸相似性为91.6%～96.9%,氨基酸序列的同源性为83.4%～96.9%.系统进化分析表明,AH-101和PD-101与原型株HPRS-103的亲缘关系最近,SZK-101与美国株AF247391的亲缘关系最近. |
| 关 键 词： | 禽白血病病毒 J亚群 血管瘤 分离 鉴定 |
Isolation and Identification of Avian Leukosis Virus Subtype J Inducing Hemangioma in Layers |
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| HOU Xinhua,SHEN Maoxin,LU Xinlun,CHU Dianfen,LIU Dong,SUN Jian,ZUO Qingshan,DU Yuanzhao.Isolation and Identification of Avian Leukosis Virus Subtype J Inducing Hemangioma in Layers[J].China Poultry,2011,33(3). | |
| Authors: | HOU Xinhua SHEN Maoxin LU Xinlun CHU Dianfen LIU Dong SUN Jian ZUO Qingshan DU Yuanzhao |
| Institution: | HOU Xinhua1,2,SHEN Maoxin1,LU Xinlun1,CHU Dianfeng2,LIU Dong2,SUN Jian2,ZUO Qingshan2,DU Yuanzhao2(1.Key Open Laboratory of Animal Infectious Diseases,Ministry of Agricultural,Yangzhou University,Yangzhou,Jiangsu 225009,2.YEBIO Bioengineering Co.,Ltd.of Qingdao,Qingdao,Shandong 266032) |
| Abstract: | In this study three strains of avian leukosis virus subgroup J(ALV-J) named AH-101,PD-101 and SZK-101 were isolated and identified by pathological anatomy,DF-1 cell culture,IFA and PCR from parent breeders in Anhui province and commercial layers in Pingdu and Shazikou of Qingdao.Blood,livers and spleens of sick chickens were collected.Pathological materials were inoculated onto DF-1 cells,the results of PCR using primers PJ1/PJ2 showed that three strains belonged to ALV-J.And IFA detection were positive.Pri... |
| Keywords: | avian leukosis virus subtype J hemangioma isolation identification |
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