| 鹅副粘病毒实时荧光定量RT-PCR检测方法的建立 |
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| 引用本文： | 张桐源,林颖,耿庆华,龙联,林书铭,赵玉军.鹅副粘病毒实时荧光定量RT-PCR检测方法的建立[J].现代畜牧兽医,2011(5):63-65. |
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| 作者姓名： | 张桐源 林颖 耿庆华 龙联 林书铭 赵玉军 |
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| 作者单位： | 1. 沈阳农业大学,辽宁,沈阳,110866
2. 沈阳出入境检验检疫局,辽宁,沈阳,110016 |
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| 摘 要： | 根据CenBank所载鹅副粘病毒(CPMV)的F基因保守区序列设计一对特异性引物,应用RT-PCR方法扩增F基因片段,克隆、测序后,采取Taqman探针法建立了检测鹅副粘病毒的荧光RT-PCR方法.结果表明,该方法特异性强,敏感性高,可以用于鹅副粘病毒的检测.
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| 关 键 词： | 鹅副粘病毒 Taqman探针法 荧光RT-PCR |
Real-time quantitative RT-PCR assay for detection of Goose paramyxo virus |
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| Zhang Tongyuan,Lin Ying,Geng Qinghua,Long Zhen,Lin Shuming,Zhao Yujun.Real-time quantitative RT-PCR assay for detection of Goose paramyxo virus[J].Modern JOurnal of Animal Husbandry and Veterinary Medicine,2011(5):63-65. |
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| Authors: | Zhang Tongyuan Lin Ying Geng Qinghua Long Zhen Lin Shuming Zhao Yujun |
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| Institution: | Zhang Tongyuan1,Lin Ying2,Geng Qinghua2,Long Zhen1,Lin Shuming2,Zhao Yujun1*(1.Shenyang Agriculture University,LiaoNing ShenYang 110866,2.Shenyang Entry-Exit Inspection and Quarantine Bureau,LiaoNing ShenYang 110016) |
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| Abstract: | One pair of primers specific based on the conservative reigon of GPMV F designed gene sequences in GenBank was designed.F gene was amplified by RT-PCR,and then cloned and sequenced.The real-time RT-PCR method was established by using the technology of Taqman probe in order to detect Goose paramyxovirus.The result showed that the method described here with highly specificity and sencificity,which could be used for detection of GPMV. |
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| Keywords: | Goose paramyxo virus Taqman probe method Fluorescence RT-PCR |
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