伪狂犬病病毒Kaplan株糖蛋白gG基因的克隆与表达

本试验利用PCR技术扩增了伪狂犬病病毒（PRV）Kaplan株的糖蛋白G（gG）基因,结果显示,扩增的片段长约为1 314 bp。将gG基因克隆到原核表达载体pET-30a中,转入大肠杆菌中并经IPTG诱导表达,通过SDSPAGE蛋白电泳和Western-Blot免疫印迹分析,证实表达了分子量大小约为54 ku的特异性gG蛋白,并能被特异性抗体所识别。本研究为深入阐明PRV gG基因结构与功能及诊断试剂的研发奠定了基础。

Cloning and Expression of Glycoprotein G Gene from pseudorabies Virus Kaplan Strain

The glycoprotein G （gG） gene of pseudorabies virus Kaplan strain was amplified by PCR followed by sequence analysis. The results revealed that the gene fragment was 1314 base pairs （bp） in length. The full-length gG gene was cloned into the prokaryotic expression vector pET-30a（＋） , and expressed in E.coli induced by IPTG. The molecular weight of the gG protein was approximately 54ku identified by SDS-PAGE and Western-Blot.The results are helpful for investigat-ing the structure and function of gG protein and preparation of diagnostic reagents for pseudora-bies.