| 钨酸钠-磷钼酸比色分光光度法测定饲料中的单宁 |
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| 引用本文： | 潘娟,商军,黄士新.钨酸钠-磷钼酸比色分光光度法测定饲料中的单宁[J].饲料工业,2012,33(7):51-55. |
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| 作者姓名： | 潘娟 商军 黄士新 |
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| 作者单位： | 上海市兽药饲料检测所,上海,201103 |
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| 摘 要： | 试验研究了用丙酮溶液提取饲料中单宁,溶液经过滤后,取滤液加钨酸钠-磷钼酸混合溶液,显色后,在760 nm波长处测定吸光度值,标准曲线法测定饲料中单宁含量。单宁在0.50～6.00 mg/l浓度范围内与吸光度值呈良好的线性关系,线性回归方程为A=0.056 48×C+0.001 08,相关系数为0.999 7,方法的检出限为17.5 mg/kg。该方法操作简便、快速、结果准确,应用于饲料中单宁的测定,结果满意。
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| 关 键 词： | 比色分光光度法 单宁测定 饲料 |
Determination of tannin in feed using spectrophotometric method with sodium tungstate- phosphomolybdic acid |
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| Pan Juan
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Shang Jun
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Huang Shixin.Determination of tannin in feed using spectrophotometric method with sodium tungstate- phosphomolybdic acid[J].Feed Industry,2012,33(7):51-55. |
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| Authors: | Pan Juan Shang Jun Huang Shixin |
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| Institution: | Pan Juan,Shang Jun,Huang Shixin |
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| Abstract: | The optimum condition of determination of tannin in feeds using spectrophotometric method with sodium tungstate-molybdphosphoric acid was studied.The linear range of the method for determination of tannin was from 0.50 to 6.00 mg/l and the limit of detection was 17.5 mg/kg.The method was simple,rapid and reliable for the determination of tannin in feeds. |
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| Keywords: | spectrophotometry method determination of tannin feeds |
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