动物组织中磺胺二甲嘧啶残留检测ELISA试剂盒的研制

在建立竞争ELISA方法的基础上，首次研制出检测磺胺二甲嘧啶的单克隆抗体快速检测试剂盒，并对其检测限、精密度、检测范围以及鸡肌肉组织中的添加回收实验做了详细研究。本试剂盒的检测限为1．0ng／m1，检测范围为1．0-81．0ng／m1，批内变异系数＜8．9％，批间变异系数＜9．5％，在10、60和200ng／m1水平鸡肌肉组织中添加，回收率为64．5％-85．5％，变异系数为6．0％-18．6％。与同类相关德国产试剂盒相比较，阳性符合率为100％。

Development of an enzyme linked immunosorbent assay kit for sulfamethazine in animal tissues

A sensitive and specific ELISA for SM2was established by anti-sulfamethazine monoclonal antibody. In the assay,sulfamethazine residue in sample was allowed to compete with the sulfamethazine-OVA conjugate immobilized on microtiter wells for binding to a limited amount of the anti-sulfamethazine monoclonal antibody, Horseradish peroxidase coupled to the goat IgG of anti-mouse IgG was selected as a tracer. Standard curves were constructed over 1 - 81 ng/ml of sulfamethazine,and the limit of detection of the ELISA was 1 ng/ml. Recoveries of sulfamethazine in spiked samples between 10, 60 and 200 ng/ml ranged from 64. 5% to 85. 5%. Precision of the assay as determined by interassay and intraassay variabilities was below 10%. Among 6 sulfonamide analogues tested for specificity .Sulfadimethoxine and sulfamer-azine showed significant cross-reaction in the assay. The assay is rapid,sensitive,and convenient to perform, and the test is readily adaptable to high-volume testing of sample in slaughterhouses.