鸭疫里默氏杆菌免疫原性研究Ⅱ.荚膜提取物免疫原性测定

本研究对Ⅰ型鸭疫里默氏杆菌的荚膜提取物的免疫原性进行了研究，结果表明，荚膜粗提物和经过苯酚抽提纯化后的荚膜提取物经２次免疫７日龄北京鸭后对同源细菌的攻毒保护率分别为９０％和７０％；采用ＥＬＩＳＡ检测荚膜提取物免疫后抗体产生规律显示，二者刺激机体产生的抗体水平有一定的差异，荚膜粗提物免疫组抗体水平高于纯化后的免疫组，前者抗体下降速度较后者慢。

Studies on the Immunogenicity of Riemerella anatipestifer Ⅱ.Observation of Immunogenicity of Capsular Material from Riemerella anatipestifer

The immunogenecity of the crude and purified capsular material of Riemeralla anatipestifer for ducklings was investigated in this study.Both evoked the antibody response, but the antibody titer of the group immunized with crude capsular material was higher than that of the purified group detected by ELISA. The protective rate against the challenge of homogenous isolate was 90% and 70% respectively after second immunization.The results demonstrated that the capsular material was immunogenic to ducklings,but its immunogenic and protective effect was related to the protein content in capsular products to some extent.