| 金丝桃素对口蹄疫病毒的体外抗病毒实验 |
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| 引用本文： | 王曙阳,梁剑平,杨林西,崔颖,尚若峰,罗永江.金丝桃素对口蹄疫病毒的体外抗病毒实验[J].中国兽医杂志,2006,42(6):6-9. |
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| 作者姓名： | 王曙阳 梁剑平 杨林西 崔颖 尚若峰 罗永江 |
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| 作者单位： | 1. 兰州大学基础医学院生物化学与分子生物学研究所,甘肃,兰州,730000
2. 中国农业科学院兰州畜牧与兽药研究所,甘肃,兰州,730000 |
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| 摘 要： | 本文报道了金丝桃素体外抗口蹄疫病毒（FMDV）的研究结果。采用双抗体捕获抗原法、病毒滴度测定、MTT法、组织细胞培养等方法均检测到抗病毒作用。结果显示：金丝桃素在BHK-21细胞感染FMDV前、后及同时加入，都有明显抑制细胞病变的作用。金丝桃素使FMDV的TCID50从8．5下降到6．25。双抗体捕获抗原法检测到金丝桃素有效抑制FMDVBHK-21细胞的吸附、融合，抑制率可达59．72％。研究结果提示：金丝桃素在体外有抗口蹄疫病毒作用，抑制口蹄疫病毒与宿主细胞的吸附、融合是抗病毒的重要途径之一。
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| 关 键 词： | 金丝桃素 抗口蹄疫病毒 BHK-21细胞 细胞病变 |
| 文章编号： | 0529-6005（2006）06-0006-04 |
| 收稿时间： | 01 25 2005 12:00AM |
| 修稿时间： | 2005年1月25日 |
Studies on the inhibitory effect of hypericin on foot-and-mouth virus in vitro |
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| WANG Shu-yang,LIANG Jian-ping,YANG Lin-xi,CUI Ying,SHANG Ruo-feng,LUO Yong-jiang.Studies on the inhibitory effect of hypericin on foot-and-mouth virus in vitro[J].Chinese Journal of Veterinary Medicine,2006,42(6):6-9. |
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| Authors: | WANG Shu-yang LIANG Jian-ping YANG Lin-xi CUI Ying SHANG Ruo-feng LUO Yong-jiang |
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| Institution: | 1. Institute of medical biochemistry and molecular-biology of Lan Zhou University, GanSu Lan Zhou 730000, China; 2. Lan Zhou Institute of Animals Science and Veterinary Pharmaceutics of CAAS, Lan zhou 730050, chain |
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| Abstract: | The inhibitory effect of hypericin on foot-and-mouth virus(FMDV) in vitro was reported in this paper.The inhibitory effect of hypericin on FMDV was examined using double-antibady trapped ELISA,neutralization test(NT),MTT colorimetric assay and tissue culture.Hypricin has significantly inhibited cytopathic effects in vitro with TCID_(50) of FMDV decreased from 8.5 to 6.25.It also strongly inhibited the adsorptive ability of FMDV to BHK-21 cells.The maximal inhibitory rate was 59.72%.These results indicate that hypericin can effectively inhibit FMDV in vitro and the inhibitory effects of hypericin on the adsorptive ability of FMDV play an important role in the mechanism of anti-FMDV. |
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| Keywords: | hypericin anti-FMDV BHK-21 cell cytopathic effect |
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