| 北京郊区猪戊型肝炎流行初步调查 |
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| 引用本文： | 李靖,刘波,杨君敬,田德雨,凌勇,何诚.北京郊区猪戊型肝炎流行初步调查[J].中国兽医杂志,2012,48(6):6-9. |
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| 作者姓名： | 李靖 刘波 杨君敬 田德雨 凌勇 何诚 |
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| 作者单位： | 中国农业大学动物医学院,北京海淀,100193 |
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| 基金项目： | 国家自然科学基金资助项目，中国科技部(MOST)“国家国际科技合作计划” |
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| 摘 要： | 用间接ELISA试剂盒对北京市郊区所采集的174份猪血清样品进行了抗体检测,结果显示,其抗体总阳性率达62.1%。针对戊型肝炎病毒(HEV)ORF2/ORF3重叠区设计了简并引物,采用巢式RT-PCR对随即采取的少量猪粪便样品进行了抗原检测。结果表明,部分粪便样品中存在HEV。
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| 关 键 词： | 猪 戊型肝炎病毒 血清流行病学 逆转录聚合酶链式反应 |
Primary epidemiological survey on the infection of hepatitis E virus in swine in Beijing suburbs |
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| LI Jing
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LIU Bo
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YANG Jun-jing
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TIAN De-yu
,
LING Yong
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HE Cheng.Primary epidemiological survey on the infection of hepatitis E virus in swine in Beijing suburbs[J].Chinese Journal of Veterinary Medicine,2012,48(6):6-9. |
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| Authors: | LI Jing LIU Bo YANG Jun-jing TIAN De-yu LING Yong HE Cheng |
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| Institution: | (College of Veterinary Medicine,China Agricultural University,Beijing 100193,China) |
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| Abstract: | To investigate hepatitis E virus(HEV) infection in pigs in Beijing suburbs,we collected 174 swine sera,and tested antibodies using ELISA kit.The results demonstrate that the total positive rate of anti-HEV antibodies was 62.1%.Responses in a nested RT-PCR assay to a pair of degenerated primers designed from ORF2/ORF3 overlapping region indicated presence of gene sequence of HEV in the stool samples. |
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