| 表没食子儿茶素没食子酸酯用于小鼠卵母细胞体外培养的效果观察 |
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| 引用本文： | 李拥军,李太坤,李文婷,张光景,谷亚轻,尚利青,邹恒.表没食子儿茶素没食子酸酯用于小鼠卵母细胞体外培养的效果观察[J].中国兽医杂志,2012,48(5):17-19. |
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| 作者姓名： | 李拥军 李太坤 李文婷 张光景 谷亚轻 尚利青 邹恒 |
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| 作者单位： | 扬州大学动物科学与技术学院江苏省动物遗传繁育与分子设计重点实验室,江苏扬州,225009 |
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| 基金项目： | 江苏高校优势学科建设工程项目，扬州大学高层次人才科研启动基金 |
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| 摘 要： | 本试验以小鼠为动物模型,采用HTF和TCM-199两个基础培养体系,通过添加不同浓度的表没食子儿茶素没食子酸酯(EGCG),研究其对小鼠卵母细胞体外成熟、体外受精及其后期胚胎发育的影响。结果表明,在HTF和TCM-199体系中,培养卵丘-卵母细胞复合物(COCs)时EGCG的最佳添加量均为20μmol/L,可以显著提高卵母细胞的成熟率、受精率和胚胎发育率。过量添加EGCG则有负面的效应。综合比较,HTF体系的培养效果优于TCM-199体系。
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| 关 键 词： | EGCG 卵母细胞 体外成熟 体外受精 胚胎发育 |
EGCG effect on in-vitro culture of mouse oocytes |
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| LI Yong-jun
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LI Tai-kun
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LI Wen-ting
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ZHANG Guang-jing
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GU Ya-qing
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SHANG Li-qing
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ZOU Heng.EGCG effect on in-vitro culture of mouse oocytes[J].Chinese Journal of Veterinary Medicine,2012,48(5):17-19. |
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| Authors: | LI Yong-jun LI Tai-kun LI Wen-ting ZHANG Guang-jing GU Ya-qing SHANG Li-qing ZOU Heng |
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| Institution: | (Key Laboratory for Animal Genetic,Breeding,Reproduction and Molecuolar Design of Jiangsu Province, College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China) |
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| Abstract: | EGCG is a natural antioxidant extracted from tea.In this study,HTF and TCM-199 were used as foundation culture systems.By adding different concentration of EGCG,EGCG effect on IVM,IVF,and embryo development of mouse oocytes was investigated.The results indicated that 20 μmol/L EGCG had the best IVC effect on maturation rate,fertility rate,and embryonic development rate both in HTF and TCM-199.Overdose of EGCG could significantly degrade IVC effect.HTF culture system was better than TCM-199. |
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| Keywords: | EGCG oocyte IVM IVF embryo debelopment |
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