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| 不同宿主来源犬瘟热病毒的分离及致病相关基因序列分析 | |
| 引用本文： | 李天松,王磊,王胜乐,姜雪,许微微,李元果,高玉伟,王铁成,黄耕,夏咸柱.不同宿主来源犬瘟热病毒的分离及致病相关基因序列分析[J].中国兽医杂志,2012,48(7):3-7. |
| 作者姓名： | 李天松 王磊 王胜乐 姜雪 许微微 李元果 高玉伟 王铁成 黄耕 夏咸柱 |
| 作者单位： | 1. 吉林农业大学动物科学技术学院,吉林长春130118;中国人民解放军军事医学科学院军事兽医研究所,吉林长春130122 2. 中国人民解放军军事医学科学院军事兽医研究所,吉林长春,130122 3. 中国人民解放军军事医学科学院军事兽医研究所,吉林长春130122;吉林省人畜共患病预防与控制重点实验室,吉林长春130122 |
| 基金项目： | 国家林业局野生动物疫病调查项目 |
| 摘 要： | 利用Vero-DST细胞从死亡犬、狐狸肺、脾内分离到4株犬瘟热病毒-CDV-TM-CC、CDV-Dog-SCh、CDV-Fox-SY、CDV-Fox-WF,在Vero-DST细胞上传5代没有细胞病变,5代细胞培养物经电镜、间接免疫荧光及PCR鉴定为阳性。与本实验室保存的CDV-Monkey-BJ进行基因测序,并对与致病相关的F蛋白、V蛋白进行分析,F蛋白分析5株毒具有6个潜在N-末端糖基化位点,与强毒株同源性在92%～99.7%,与疫苗株不高于93.3%,在F1亚基内有7个特有氨基酸位点,这可能与其对灵长类致病有关。V蛋白分析表明,其与强毒株同源性在94%～99.7%之间,而与疫苗株不高于93.3%,CDV-Monkey-BJ C272R突变使其Zn结合能力丧失。试验结果显示,所有分离株均为强毒株,不同宿主毒株序列存在差异,值得进一步研究。 |
| 关 键 词： | 犬瘟热病毒 V蛋白 F蛋白 |
The isolation and related pathogenic gene sequence analysis of canine distemper virus from different hosts |
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| LI Tian-song , WANG Lei , WANG Sheng-le , JIANG Xue , XU Wei-wei , LI Yuan-guo , GAO Yu-wei , WANG Tie-cheng , HUANG Geng , XIA Xian-zhu.The isolation and related pathogenic gene sequence analysis of canine distemper virus from different hosts[J].Chinese Journal of Veterinary Medicine,2012,48(7):3-7. | |
| Authors: | LI Tian-song WANG Lei WANG Sheng-le JIANG Xue XU Wei-wei LI Yuan-guo GAO Yu-wei WANG Tie-cheng HUANG Geng XIA Xian-zhu |
| Institution: | 2,3(1.Jilin Agriculture University,The Department of Animal Science and Technology,Jilin Chunchun 130118; 2.Military Veterinary institute,Academy of Military Medical Science of PLA,Jilin Changchun 130112;3.Institute of Military Veterinary,AMMS,Key laboratory of Jilin province for zoonosis prevention and control,Jilin Changchun 130112) |
| Abstract: | A new canine distemper virus was isolated from dead canine and fox lung and spleen using Vero-DST,which was designated as strain CDV-TM-CC、CDV-Dog-SCh、CDV-Fox-SY、CDV-Fox-WF.There were no cytopathic effect on Vero-DST through five passages.The cell cultures confirmed the results from CDV-positive by electron microscopy,indirect immunofluorescence and PCR.These genomes were sequenced,F protein has six potential N-glycosylation sites,92.3%~99.7% homology with the virulent strain,only at most 93.3% with the vaccine strain,seven special amino acid sites are located in F1 subunit,which may be related to primate pathogenicity.The V protein analysis indicated that the homology with the virulent strain is between 93.8% and 99.1%,only at most 93.3% with the vaccine strain.The mutation of C272R made CDV-Monkey-BJ lose the ability of zinc combination. |
| Keywords: | Canine distemper virus V protein F protein |
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