| 猪瘟病毒E2基因密码子优化后在昆虫细胞中的表达 |
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| 引用本文： | 范学政,徐璐,王琴,赵启祖,宁宜宝,陈锴.猪瘟病毒E2基因密码子优化后在昆虫细胞中的表达[J].中国兽医杂志,2011,47(5). |
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| 作者姓名： | 范学政 徐璐 王琴 赵启祖 宁宜宝 陈锴 |
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| 作者单位： | 中国兽医药品监察所猪瘟参考实验室,北京海淀,100081 |
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| 基金项目： | 农业部"948"项目，国家科技支撑计划 |
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| 摘 要： | 将猪瘟病毒E2基因密码子改造后,克隆入pFastBac1质粒载体,经转座、转染后构建重组杆状病毒.并对表达产物进行鉴定分析.结果:E2基因改造后与设计序列一致;成功获得了重组杆状病毒(BAC/SE2M),采用猪瘟E2单抗WH303进行Western-blot和ELISA鉴定,证明:表达的CSFV E2蛋白特异性好,纯化后的E2蛋白,可用于猪瘟抗体检测试剂盒的开发.
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| 关 键 词： | 猪瘟病毒 E2基因 密码子优化 杆状病毒 |
The optimization of CSFV E2 gene condons and expression by insect cells |
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| FAN Xue-zheng,XU Lu,WANG Qin,ZHAO Qi-zu,NING Yi-bao,CHEN Kai.The optimization of CSFV E2 gene condons and expression by insect cells[J].Chinese Journal of Veterinary Medicine,2011,47(5). |
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| Authors: | FAN Xue-zheng XU Lu WANG Qin ZHAO Qi-zu NING Yi-bao CHEN Kai |
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| Institution: | FAN Xue-zheng,XU Lu,WANG Qin,ZHAO Qi-zu,NING Yi-bao,CHEN Kai(Classical Swine Fever Reference Laboratory at China Institute of Veterinary Drug Control,Beijing 100081,China) |
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| Abstract: | The E2 gene of classical swine fever virus(CSFV) was optimized in order to be expressed in baculovirus.The modified E2 gene of CSFV,honeybee melittin signal(Mels) sequence and six-histidine tag were fused and cloned into vector pFastBac1.After transformation to DH10Bac and transfection to sf9,a recombinant virus named BAC/SE2M was constructed.The supernatant was concentrated and purified with Nie+ affinity chromatography.Purified E2 protein can be recognized by mAb WH303.Recombinant E2 protein expressed by ... |
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| Keywords: | CSFV E2 gene codon optimization BEVS |
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