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| 不同剂量的猪瘟活疫苗单次免疫效果评价及疫苗存留试验 | |
| 引用本文： | 熊丁杰,范学政,徐璐,王琴,徐志文,郭万柱,周远成,刘俊,陈蕾,汤波.不同剂量的猪瘟活疫苗单次免疫效果评价及疫苗存留试验[J].中国兽医杂志,2011,47(8). |
| 作者姓名： | 熊丁杰 范学政 徐璐 王琴 徐志文 郭万柱 周远成 刘俊 陈蕾 汤波 |
| 作者单位： | 1. 中国兽医药品监察所猪瘟参考实验室,北京海淀100081;四川农业大学动物生物技术中心,四川雅安625014 2. 中国兽医药品监察所猪瘟参考实验室,北京海淀,100081 3. 四川农业大学动物生物技术中心,四川雅安,625014 |
| 基金项目： | “十一五”国家科技支撑计划课题(2006BAD06A18,2006BAD06A03) |
| 摘 要： | 用1 200 RID、6 000 RID和24 000 RID 3种不同剂量的猪瘟活疫苗单次免疫9头30日龄的断奶仔猪,检测试验猪的抗体消长和疫苗毒核酸在猪体内的存留时间,并用6头猪进行攻毒保护试验.结果表明:不同免疫剂量的试验猪其抗体水平的动态变化没有显著差异,免疫后21 d试验猪的猪瘟抗体均成阳性,63 d后至578 d猪瘟抗体阻断率仍然高于85％,应用荧光定量RT-PCR技术检测,猪瘟疫苗毒核酸在猪血液和扁桃体中的检出时间分别达28 d和42 d;免疫后370 d进行猪瘟强毒攻击后,免疫猪没有出现明显的临床症状,存活且不带毒.本研究证实,没有其他疫病和母源抗体的仔猪单次接种合理剂量的猪瘟活疫苗就能产生坚强的保护力,提出加强种猪场各种病原监测,淘汰病原阳性种猪,净化种猪群对提高猪瘟疫苗免疫效果,最终控制猪瘟具有重要意义. |
| 关 键 词： | 猪瘟疫苗 免疫评价 残留 |
Studies on the protective effects of different doses of classical swine fever(CSF) vaccine against CSF |
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| XIONG Ding-jie,FAN Xue-zheng,XU Lu,WANG Qin,XU Zhi-wen,GUO Wan-zhu,ZHOU Yuan-cheng,LIU Jun,CHEN Lei,TANG Bo.Studies on the protective effects of different doses of classical swine fever(CSF) vaccine against CSF[J].Chinese Journal of Veterinary Medicine,2011,47(8). | |
| Authors: | XIONG Ding-jie FAN Xue-zheng XU Lu WANG Qin XU Zhi-wen GUO Wan-zhu ZHOU Yuan-cheng LIU Jun CHEN Lei TANG Bo |
| Institution: | XIONG Ding-jie1,2,FAN Xue-zheng1,XU Lu1,WANG Qin1,XU Zhi-wen2,GUO Wan-zhu2,ZHOU Yuan-cheng1,LIU Jun1,CHEN Lei1,TANG Bo1(1.China Institute of Veterinary Drug Control,National Reference Laboratory of Classical Swine Fever,Beijing 100081,China,2.Centre for Animal Biotechnology,Sichuan Agricultural University,Yaan 625014,China) |
| Abstract: | Nine weaned pigs of 30 days old were inoculated three different doses of CSFV vaccine in a single immunization.The antibody titer and the nucleic acid residues of vaccine virus were detected,and then 6 pigs were challenged.It was demonstrated that no significant difference could be observed in antibody titer of the pigs injected with different doses of CSFV vaccine.Antibodies against CSFV were transformed into positive on 21 days after vaccination and blocking rate were higher than 85% between 63 and 578 da... |
| Keywords: | CSF vaccine evaluation virus residue |
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