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| 甲型H1N1流感病毒核酸检测能力验证样品制备及其应用效果评价 | |
| 引用本文： | 乔彩霞,高志强,张利峰,蒲静,谷强,张伟,刘来福,刘金华,张鹤晓.甲型H1N1流感病毒核酸检测能力验证样品制备及其应用效果评价[J].中国兽医杂志,2011,47(8). |
| 作者姓名： | 乔彩霞 高志强 张利峰 蒲静 谷强 张伟 刘来福 刘金华 张鹤晓 |
| 作者单位： | 1. 中国农业大学动物医学院,北京海淀100193;北京出入境检验检疫局,北京朝阳100026 2. 北京出入境检验检疫局,北京朝阳,100026 3. 中国农业大学动物医学院,北京海淀,100193 |
| 基金项目： | 国家科技基础性工作专项“重大动物疫病病原及相关制品标准物质的研究”(2008FY130100); 国家标准专项“动物流感检测技术标准体系的研制” |
| 摘 要： | 为考核实验室检测甲型H1N1流感病毒的能力,设计和实施了“CNAS T0459甲型H1N1流感病毒核酸检测能力验证计划”.利用甲型H1N1流感病毒(2009)和经典H1N1猪流感病毒核酸标准物质制备6组能力验证阳性样品,经实验室检测分析,所制备的样品均匀性和稳定性均达到中国合格评定国家认可委员会对能力验证样品的要求.将6组阳性样品和1组阴性样品编号后下发54家参试实验室,共有50家实验室报送了有效数据,其中完全达到能力验证要求的实验室共34家,满意率达68％,其余16家为不满意实验室,包括8家实验室检测经典H1N1猪流感病毒满意,但甲型H1N1流感病毒(2009)特异性检测不满意.本次能力验证为整体提升我国甲型H1N1流感病毒的检测水平和评估各级实验室检测能力具有重要意义. |
| 关 键 词： | 甲型H1N1流感病毒 经典H1N1猪流感病毒 核酸检测 能力验证 |
Development and application evaluation of reference samples for proficiency testing program for 2009 influenza A (H1N1) virus nucleic acid detection |
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| QIAO Cai-xia,GAO Zhi-qiang,ZHANG Li-feng,PU Jing,GU Qiang,ZHANG Wei,LIU Lai-fu,LIU Jin-hua,ZHANG He-xiao.Development and application evaluation of reference samples for proficiency testing program for 2009 influenza A (H1N1) virus nucleic acid detection[J].Chinese Journal of Veterinary Medicine,2011,47(8). | |
| Authors: | QIAO Cai-xia GAO Zhi-qiang ZHANG Li-feng PU Jing GU Qiang ZHANG Wei LIU Lai-fu LIU Jin-hua ZHANG He-xiao |
| Institution: | QIAO Cai-xia1,2,GAO Zhi-qiang2,ZHANG Li-feng2,PU Jing2,GU Qiang2,ZHANG Wei2,LIU Lai-fu2,LIU Jin-hua1,ZHANG He-xiao2(1.College of Veterinary Medicine,China Agricultural University,Beijing 100193,China,2.Beijing Enrty-exit Inspection and Quarantine Bureau,Beijing 100026,China) |
| Abstract: | The CNAS T0459,Proficiency testing program for detection nucleic acid of 2009 influenza A(H1N1) virus,was designed and implemented to ensure reliable and recognized nucleic acid detection service for the participant labs.Standard reference materials for nucleic acid of 2009 influenza A(H1N1) virus and classical H1N1 swine influenza virus were used to prepare six positive samples for proficiency testing.Based on the homogeneity and stability testing,the quality of samples meets the needs of China national ac... |
| Keywords: | 2009 influenza A(H1N1)virus classical H1N1 swine influenza virus detection nucleic acid proficiency testing |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |