| 瑞氏木霉基因组DNA提取方法比较研究 |
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| 引用本文： | 向会耀,农向,郝葆青,秦天莺,黎世超,陈学梅.瑞氏木霉基因组DNA提取方法比较研究[J].四川草原,2006(2):16-18. |
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| 作者姓名： | 向会耀 农向 郝葆青 秦天莺 黎世超 陈学梅 |
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| 作者单位： | [1]西南民族大学生命科学与技术学院,四川成都610041 [2]广东信宜丽株动物药业有限公司,广东信宜525300 |
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| 基金项目： | 西南民族大学自然科学基金资助项目（项目号：23444） |
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| 摘 要： | 采用4种常规DNA提取方法提取瑞氏木霉基因组DNA。结果表明:EDTA-SDS法和冷冻研磨CTAB 法更适合此真菌DNA的提取,试剂盒提取的总DNA纯度和浓度比较高,但是价格昂贵,冷冻研磨SDS法提取的 DNA浓度不高,不适合分子生物学操作的要求。
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| 关 键 词： | 瑞氏木霉 基因组DNA 冷冻研磨CTAB法 EDTA-SDS法 冷冻研磨SDS法 |
| 文章编号： | 1006-1932（2006）02-0016-03 |
| 收稿时间： | 2005-10-12 |
Comparison of the Methods of Genomic DNA Extraction from Trichoderma Reesei |
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| XIANG Hui-yao, NONG Xiang, HAO Bao-qing, QIN Tian-ying, LI Shi-chao, CHEN Xue-mei .Guangdortg xinyi lizhu animal Pharmaceutical co., LTD, Xinyi , China.Comparison of the Methods of Genomic DNA Extraction from Trichoderma Reesei[J].Journal of Sichuan Grassland,2006(2):16-18. |
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| Authors: | XIANG Hui-yao NONG Xiang HAO Bao-qing QIN Tian-ying LI Shi-chao CHEN Xue-mei Guangdortg xinyi lizhu animal Pharmaceutical co LTD Xinyi China |
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| Institution: | 1.College of life science and technology, southwest unive rs ity for nationalities, Chengdu 610041, China; |
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| Abstract: | In order to obtain an ideality genomic DNA template for molecule-biology research, Author used four methods to extract genomic DNA From trichoderma, the results showed that:EDTA-SDS method and refrigeration-rubbing CTAB method are suitable to extract gemomic DNA from Trichoderma, kit method extract DNA with high purity and yield but very expensive, refrigeration-rubbing SDS method extract a low yield of DNA not suitable for molecule-biology experiments. |
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| Keywords: | Trichoderma reesei Genomic DNA Refrigeration-rubbing CTAB method EDTA-SDS method Refrigeration-rubbing SDS |
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