| 柞蚕蛹血淋巴多酚氧化酶活性影响因素的研究 |
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| 引用本文： | 郑启凯,刘金波,陈思,刘限.柞蚕蛹血淋巴多酚氧化酶活性影响因素的研究[J].北方蚕业,2011,32(4):34-36,39. |
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| 作者姓名： | 郑启凯 刘金波 陈思 刘限 |
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| 作者单位： | 沈阳农业大学生物科学技术学院,辽宁沈阳,110866;沈阳农业大学生物科学技术学院,辽宁沈阳,110866;沈阳农业大学生物科学技术学院,辽宁沈阳,110866;沈阳农业大学生物科学技术学院,辽宁沈阳,110866 |
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| 基金项目： | 国家现代农业产业技术体系建设专项;辽宁省昆虫资源工程技术中心专项 |
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| 摘 要： | 柞蚕血淋巴中多酚氧化酶活性的大小对柞蚕丝产量有一定影响,并对柞蚕蛹色起着非常重要的作用。本试验采用分光光度计法对柞蚕蛹血淋巴多酚氧化酶活性大小的影响因素进行了研究。结果表明:柞蚕蛹血淋巴多酚氧化酶的最适pH值为5.0;最适温度为25℃;少量的苯基硫脲对其的抑制作用较为明显;不同化合物对多酚氧化酶的活性影响不同,在相同浓度下,氯化钙和氯化镁对柞蚕蛹血淋巴多酚氧化酶的活性有促进作用,其他化合物(EDTA,维生素C和亚硫酸钠等)则表现为抑制作用。
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| 关 键 词： | 柞蚕蛹 血淋巴 多酚氧化酶 活性 |
Study on Affecting Factors of the Activity of Haemolymph Polyphenol Oxidase for Tussah Pupa |
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| ZHENG Qikai, LIU Jinbo, CHEN Si, LIU Xian.Study on Affecting Factors of the Activity of Haemolymph Polyphenol Oxidase for Tussah Pupa[J].North Sericulture,2011,32(4):34-36,39. |
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| Authors: | ZHENG Qikai LIU Jinbo CHEN Si LIU Xian |
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| Institution: | * (College of Animal Science and Technology, Shenyang Agriculture University, Shenyang Liaoning 110866,China) |
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| Abstract: | The Polyphenol oxidase (PPO) in haemolymph of tussah pupa exerted a great impact on the yield of tussah silk as well as the color.The activity of polyphenol oxidase in haemolymph of tussah pupa and its factors were studied with spectrophotometer. The results showed that the optimal pH of polyphenol oxidase was 5.0 and the optimum temperature was 25℃; Small amounts of phenyl thiourea could inhibited obviously the activity of PPO; Different compounds have different effects on the activity of PPO; NaCl and MgCl2 could improve the activity of polyphenol oxidase,whereas other compounds showed the inhibitory effect, such as EDTA, Vc and sodium sulfite etc. |
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| Keywords: | Tussah pupa haemolymph Polyphenol oxidase activity |
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