Bovine CD4 T-cell lines reactive with soluble and membrane antigens of Cowdria ruminantium |
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Authors: | P Tott J Nyanjui A Bensaid D McKeever |
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Institution: | P. Totté, J. Nyanjui, A. Bensaid,D. McKeever |
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Abstract: | Cowdria-specific CD4+ T-cell lines generated from immunised cattle respond to both soluble and membrane proteins of the agent. Furthermore, the lines produced the Cowdria-inhibitory cytokine IFN-γ in response to soluble antigens fractionated by gel filtration and FPLC. Activity eluted as a single peak around fraction 15 for all T-cell lines tested. This fraction induced the highest production of IFN-γ by the lines and was shown by SDS-polyacrylamide gel electrophoresis and silver staining analysis to contain less than 10 different bands ranging from 22 to 32 kDa. Given their high sensitivity and specificity, these short-term CD4+ T-cell lines will be valuable tools for the identification of Cowdria antigens for incorporation in a subunit vaccine. |
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Keywords: | Cowdria ruminantium CD4+ T cell lines IFN-γ Immunogenic proteins Fast performance liquid chromatography (FPLC) |
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