Immunostimulatory effects of natural human interferon-alpha (huIFN-α) on carps Cyprinus carpio L. |
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Authors: | Hironobu Watanuki Gunimala Chakraborty Hiroki Korenaga Tomoya Kono RB Shivappa Masahiro Sakai |
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Institution: | aFaculty of Agriculture, University of Miyazaki, Gakuen Kibanadai Nishi 1-1, Miyazaki 889-2192, Japan;bCollege of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA |
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Abstract: | Human interferon-α (huIFN-α) is an important immunomodulatory substance used in the treatment and prevention of numerous infectious and immune-related diseases in animals. However, the immunostimulatory effects of huIFN-α in fish remain to be investigated. In the current study, the immune responses of the carp species Cyprinus carpio L. to treatment with huIFN-α were analyzed via measurement of superoxide anion production, phagocytic activity and the expression of cytokine genes including interleukin-1β, tumor necrosis factor-α and interleukin 10. Low doses of huIFN-α were administered orally once a day for 3 days, and sampling was carried out at 1, 3 and 5 days post-treatment. Our results indicate that a low dose of huIFN-α significantly increased phagocytic activity and superoxide anion production in the carp kidney. The huIFN-α-treated fish also displayed a significant upregulation in cytokine gene expression. The current study demonstrates the stimulatory effects of huIFN-α on the carp immune system and highlights the immunomodulatory role of huIFN-α in fish. |
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Keywords: | huIFN-α Phagocytic activity Cytokine genes Fish |
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