| 家鸭×番鸭杂种下调表达新基因r-MHD2的克隆 |
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| 引用本文： | 单雪松,陈洋,崔庆媛,李克鹏.家鸭×番鸭杂种下调表达新基因r-MHD2的克隆[J].家畜生态学报,2009,30(4). |
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| 作者姓名： | 单雪松 陈洋 崔庆媛 李克鹏 |
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| 作者单位： | 吉林农业大学动物科学技术学院,吉林,长春,130118 |
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| 摘 要： | 以北京鸭、法国番鸭及其正反交F1代杂种为试验材料,采用mRNA差异显示(DDRT-PCR)方法分析不同试验组肌肉组织基因表达差异,结合抑制性消减杂交(SSH)加以验证,发现一个杂种下调表达基因r-MHD2,对鸭r-MHD2基因698 bp片段BLASTING结果表明,该基因与鸡(Gallus gallus)、非洲爪蟾Xenopus laevis和Xenopus tropicalis同源性分别为91%、78%和77%.r-MHD2基因的具体分子功能尚属未知.
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| 关 键 词： | MHD2基因 杂种优势 mRNA差异显示 鸭 番鸭 |
Cloning of a Hybrid Down-regulated Gene r-MHD2 in Domestic Duck and Muscovy Duck Crossbreeding System |
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| SHAN Xue-song,CHEN Yang,CUI Qing-yuan,LI Ke-peng.Cloning of a Hybrid Down-regulated Gene r-MHD2 in Domestic Duck and Muscovy Duck Crossbreeding System[J].Acta Ecologae Animalis Domastici,2009,30(4). |
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| Authors: | SHAN Xue-song CHEN Yang CUI Qing-yuan LI Ke-peng |
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| Abstract: | The gene expression of muscle tissue from Beijing duck, France muscovy duck and their mutual hybrids were examined with the method of DDRT-PCT, and the display result was verified by the method of SSH.One hybrid down-regulated gene r-MHD2 was found in this research.The sequencing and blasting result of the 698bp fragment of duck r-MHD2 gene showed that the identity of this gene and Gallus gallus, Xenopus laevis and Xenopus tropicalisgene is 91%, 78% and 77% respectively. |
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| Keywords: | MHD2 gene hybrid vigor mRNA differential display duck muscovy duck |
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