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| Tn5转座子介导表达猪瘟E2蛋白和EGFP重组伪狂犬病毒的构建 | |
| 引用本文： | 高博,范志强,韩乃君,张念,张锦霞,王颖,白安斌,黄红梅,扈荣良,吴健敏.Tn5转座子介导表达猪瘟E2蛋白和EGFP重组伪狂犬病毒的构建[J].中国兽医寄生虫病,2011,19(3):14-18. |
| 作者姓名： | 高博 范志强 韩乃君 张念 张锦霞 王颖 白安斌 黄红梅 扈荣良 吴健敏 |
| 作者单位： | 1. 广西大学,南宁,530005;军事医学科学院军事兽医研究所,长春,130122;广西兽医研究所,南宁,530001 2. 军事医学科学院军事兽医研究所,长春,130122 3. 广西兽医研究所,南宁,530001 |
| 摘 要： | 为构建猪瘟重组伪狂犬疫苗,以Tn5转座子为介导,采用体外转座的方法将猪瘟病毒（Classical swine fever virus,CSFV）E2基因和增强绿色荧光蛋白（enhanced green fluoresce protein,EGFP）的表达盒随机插入伪狂犬病毒（Pseudorabies virus,PRV）基因组中,随后将转座产物转染BHK-21细胞,获得表达E2蛋白和EGFP的病毒库,并对E2蛋白和EGFP在细胞上的表达效果进行PCR鉴定和间接免疫荧光分析。试验结果表明,运用体外转座的方法可以将外源基因插入PRV基因组中,并且外源基因可以在细胞中表达。本研究为重组病毒的研究提供了新思路,并为新型重组猪瘟疫苗的研制进一步奠定了基础。 |
| 关 键 词： | 猪瘟病毒 E2基因 Tn5转座子 绿色荧光蛋白 伪狂犬病毒 |
CONSTRUCTION OF THE RECOMBINANT PRV EXPRESSING E2 AND EGFP PROTEIN BY TN5 TRANSPONSON |
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| GAO Bo , FAN Zhi-qiang , HAN Nai-jun , ZHANG Nian , ZHANG Jin-xia , WANG Ying , BAI An-bin , HUANG Hong-mei , HU Rong-liang , WU Jian-min.CONSTRUCTION OF THE RECOMBINANT PRV EXPRESSING E2 AND EGFP PROTEIN BY TN5 TRANSPONSON[J].Chinese Journal of Veterinary Parasitology,2011,19(3):14-18. | |
| Authors: | GAO Bo FAN Zhi-qiang HAN Nai-jun ZHANG Nian ZHANG Jin-xia WANG Ying BAI An-bin HUANG Hong-mei HU Rong-liang WU Jian-min |
| Institution: | GAO Bo1,2,3,FAN Zhi-qiang2,HAN Nai-jun2,ZHANG Nian3,ZHANG Jin-xia2,WANG Ying2,BAI An-bin3,HUANG Hong-mei3,HU Rong-liang2,WU Jian-min3(1.Guangxi University,Nanning 530005,China,2.Veterinary Research Institute,Academy of Military Medical Sciences,Changchun 130122,3.Guangxi Veterinary Research Institute,Nanning 530001,China) |
| Abstract: | In this study,the ORF containing E2 gene of the Classical swine fever virus(CSFV) and the enhanced green fluoresce protein(EGFP) gene were inserted randomly into the Pseudorabies virus(PRV) genome by Tn5 transponson in vitro.Then the transposition product was transfected into BHK-21 cells to generate the recombinant PRV.Finally,the recombinant PRV was identified by PCR and indirect immunofluorescence assay.The results indicated that the target genes were inserted into the PRV genome successfully and express... |
| Keywords: | Classical swine fever virus(CSFV) E2 gene Tn Transponson enhanced green fluoresce protein(EGFP) Pseudorabies virus(PRV) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |