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| 大肠杆菌热敏肠毒素B亚基的原核表达及生物学活性分析 | |
| 引用本文： | 王会,何孔旺,陆承平.大肠杆菌热敏肠毒素B亚基的原核表达及生物学活性分析[J].中国兽医寄生虫病,2011,19(2):31-36. |
| 作者姓名： | 王会 何孔旺 陆承平 |
| 作者单位： | 1. 山东省枣庄市畜牧兽医局,枣庄,277100 2. 江苏省农科院兽医研究所,南京,210014 3. 南京农业大学,南京,210095 |
| 摘 要： | 从大肠杆菌K88（LT＋,ST＋）菌株中扩增热敏肠毒素B亚基基因（ltb）,得到454 bp片段,克隆至pMD18-T载体后,与pET32a（＋）定向连接,并转化入大肠杆菌BL21中,用IPTG 30℃诱导表达重组LTB蛋白。SDS-PAGE显示,重组蛋白分子量约为34 kDa,超声波裂解后,表达产物主要以包涵体形式存在。经鉴定,纯化复性后的LTB蛋白保留部分与GM1结合的生物学活性。 |
| 关 键 词： | ltb基因 原核表达 生物学活性 |
PROKARYOTIC EXPRESSION OF ESCHERICHIA COLI HEAT-LABILE ENTEROTOXIN SUBUNIT B AND ITS BIO-ACTIVITY ANALYSIS |
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| WANG Hui,HE Kong-wang,LU Cheng-ping.PROKARYOTIC EXPRESSION OF ESCHERICHIA COLI HEAT-LABILE ENTEROTOXIN SUBUNIT B AND ITS BIO-ACTIVITY ANALYSIS[J].Chinese Journal of Veterinary Parasitology,2011,19(2):31-36. | |
| Authors: | WANG Hui HE Kong-wang LU Cheng-ping |
| Institution: | WANG Hui1,HE Kong-wang2,LU Cheng-ping3(1.Animal Husbandry and Veterinary Bureau of Zaozhuang,Shangdong Province,Zaozhuang 277100,China,2.Institute of Veterinary Medicine JAAS,Nanjing 210014,3.Nanjing Agricultural University,Nanjing 210095,China) |
| Abstract: | The gene of Escherichia coli heat-labile enterotoxin subunit B was cloned into plasmid pET32a vector and transformed into E.coli BL21.The recombinant protein was expressed after being induced by IPTG at 30℃.The SDS-PAGE indicated that the molecular weight of recombinant LTB was about 34 kDa.Most of the recombinant protein existed in inclusion body after ultrasonication,and part of protein regained bio-activity of binding with GM1 after renaturation. |
| Keywords: | LTB gene prokaryotic expression bio-activity |
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