Exogenous paraoxonase‐1 during oocyte maturation improves bovine embryo development in vitro |
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| Authors: | JAA Rincón EM Madeira FT Campos B Mion JF Silva VA Absalón‐Medina WR Butler MN Corrêa LMC Pegoraro A Schneider |
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| Institution: | 1. Department of Veterinary Clinics, Veterinary College, Universidade Federal de Pelotas, Pelotas, Brazil;2. Department of Pathology, Veterinary College, Universidade Federal de Pelotas, Pelotas, Brazil;3. Department of Clinical Studies Section of Reproduction and Behavior, School of Veterinary Medicine, University of Pennsylvania New Bolton Center, Kennett Square, PA, USA;4. Department of Animal Science, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY, USA;5. Empresa Brasileira de Pesquisa Agropecuária (Embrapa) Clima Temperado, Pelotas, Brazil;6. Department of Nutrition, College of Nutrition, Universidade Federal de Pelotas, Pelotas, Brazil |
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| Abstract: | Paraoxonase‐1 (PON1) is an enzyme found in serum and follicular fluid that protects cell membrane and circulating lipids against oxidative damage. The aims of this study were to measure the direct effects of recombinant PON1 (rPON1) on bovine oocyte maturation at the molecular level (gene expression) and to measure the carry‐over effects of PON1 on pre‐implantation embryo development in vitro. COCs were submitted to IVM with the addition of 0.0, 0.02, 0.04 and 0.08 mg ml?1 of rPON1, corresponding to an average PON1 arylesterase enzyme activity of 2.2 ± 0.4, 15.5 ± 1.5, 30.2 ± 3.0 and 57.9 ± 5.0 U ml?1, respectively. The results indicated that addition of rPON1 during IVM improved embryo development in a dose‐dependent manner as D7 embryo development was 22.2%, 29.4%, 32.2% and 37.0% for the treatment groups, respectively (p = 0.02). In conclusion, addition of PON1 enzyme during IVM exerted dose‐related positive effects on embryo development rates to blastocysts. |
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