Effect of bovine seminal plasma on bovine endometrial epithelial cells in culture |
| |
| Authors: | T Nongbua Y Guo A Edman P Humblot JM Morrell |
| |
| Institution: | 1. Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden;2. Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham, Thailand;3. VikingGenetics, Skara, Sweden |
| |
| Abstract: | The purpose of this study was to investigate the effect of seminal plasma (SP) from bulls of known fertility on bovine endometrial epithelial cells (bEEC) in culture. The bEEC from passage 5, approximately 5.0–13 × 105 cells per flask, were challenged with SP from bulls of high or low fertility (n = 3 and 2, respectively) or PBS (control), at 1% (75 μl) or 4% (300 μl) and were incubated for 72 hr (n = 13 per challenge). Total cell number and viability of bEEC after challenge with 1% SP from either high‐ or low‐fertility bulls (75H or 75L, respectively) did not differ from controls. In contrast, challenge with 4% of SP from high‐ or low‐fertility bulls (300H or 300L) negatively affected bEEC cell number and viability. Challenge with 300 L had a greater adverse effect than 300H. These results suggest that the negative effect of bovine SP on bEEC is both dose‐dependent and fertility‐dependent. |
| |
| Keywords: | bovine fertility seminal plasma uterine cell |
|
|
