Development of a one-step strip test for the diagnosis of chicken infectious bursal disease |
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Authors: | Zhang Gai-Ping Li Qing-Mei Yang Yan-Yan Guo Jun-Qing Li Xue-Wu Deng Rui-Guang Xiao Zhi-Jun Xing Guang-Xu Yang Ji-Fei Zhao Dong Cai Shu-Jun Zang Wei-Min |
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Institution: | Institute of Biotechnology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. |
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Abstract: | A rapid diagnostic strip for chicken infectious bursal disease (IBD) was developed based on membrane chromatography using high-affinity monoclonal antibodies directed to chicken infectious bursal disease virus (IBDV). The diagnostic strip has high specificity for detection of chicken IBDV antigen and recognizes a variety of the virus isolates, including virulent and attenuated strains, with no cross-reactivity to other viruses, such as Newcastle disease virus, Marek's disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, and egg-drop-syndrome virus. The results showed that its specificity was highly consistent with the agar-gel precipitation test (AGP). The diagnostic strip detected as low as 800 median egg lethal dose (ELD50) viruses in the IBDV BC6/85-infected sample, which was comparable with AC-ELISA (400 ELD50) and 32 times more sensitive than the AGP test (2.56 x 10(4) ELD50). In experimental infection, IBDV was detected in the bursa as early as 36 hr postinfection with the diagnostic strip before the clinical signs and gross lesions appeared. It takes only 1-2 min to do a strip test to detect chicken IBDV antigen after the specimen is grounded in a whirl pack with finger massage. |
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