Biochemical and Antioxidant Changes in Plasma, Serum, and Erythrocytes of Horses before and after a Jumping Competition |
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Authors: | Julio Cesar Mendes Soares Ricardo Zanella Carlos Bondan Leonardo Porto Alves Marina Ragagnin de Lima Adriana Costa da Motta Eraldo Lourenso Zanella |
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Institution: | a Universidade Federal do Pampa, Campus Itaqui, Rio Grande do Sul, Brasil b Department of Animal Sciences, Washington State University, Pullman, WA c Faculdade de Agronomia e Medicina Veterinária, Curso de Medicina Veterinária, Universidade de Passo Fundo, Passo Fundo, Rio Grande do Sul, Brasil d Laboratório de Patologia Animal, Faculdade de Agronomia e Medicina Veterinária, Curso de Medicina Veterinária, Universidade de Passo Fundo, Passo Fundo, Rio Grande do Sul, Brasil |
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Abstract: | We designed an experiment to determine the concentrations of a marker of lipid peroxidation in erythrocytes of horses submitted to jumping competitions. Erythrocytes of exercised horses showed a significant increase in the concentration of thiobarbituric acid-reactive species content immediately after exercise (P < .001), which returned to normal levels 24 hours after exercise. Nonprotein sulfhydryl groups and superoxide dismutase activity (EC 1.15.1.1) in erythrocytes were significantly higher 24 hours after exercise, as compared with the resting period and control group (P < .001). Immediately after exercise, horses had increased serum concentrations of uric acid (P < .002) and plasma lactate, as well as increased creatine kinase (EC 2.7.3.2) and lactate dehydrogenase (EC 1.1.1.28) activities (P < .001), as compared with resting period and control group. All parameters returned to normal values 24 hours after exercise, except for uric acid serum levels that remained increased (P < .001). We conclude that the oxidative stress in erythrocytes of exercised horses may contribute to tissue damage. In addition, our results showed that horses submitted to a jumping competition showed higher production of free radicals and as a consequence, lipid peroxidation. |
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Keywords: | Horse Jump competition Oxidative stress Lipoperoxidation Muscle damage |
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