<Emphasis Type="Italic">Arcobacter</Emphasis> spp. in fecal samples from Brazilian farmed caimans (<Emphasis Type="Italic">Caiman yacare</Emphasis>, Daudin 1802) |
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| Authors: | Maria Gabriela Xavier Oliveira Leandro Nogueira Pressinotti Giovane Spínola Carvalho Mirela Caroline Vilela Oliveira Luisa Zanolli Moreno Carlos Emilio Cabrera Matajira Alessandro Spínola Bergamo Victor Manuel Aleixo Alexandre Caixeta Veiga Elvis de Souza Corsino Ana Paula Guarnieri Christ Maria Inês Zanolli Sato Andrea Micke Moreno Terezinha Knöbl |
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| Institution: | 1.Faculdade de Medicina Veterinária e Zootecnia da Universidade de S?o Paulo,S?o Paulo,Brazil;2.Universidade do Estado de Mato Grosso,Cáceres,Spain;3.Instituto Federal de Educa??o Ciência e Tecnologia de Mato Grosso Campus Cáceres,Cáceres,Spain;4.Companhia Ambiental do Estado de S?o Paulo (CETESB),Pinheiros,Brazil |
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| Abstract: | The aim of this study was to perform the identification and molecular characterization of Arcobacter cryaerophilus and Arcobacter butzleri isolated from caiman (Caiman yacare), kept at a production farm, in Brazil. Forty fecal samples were analyzed. After isolation and identification, 21/40 strains of A. butzleri and 19/40 strains of A. cryaerophilus were subjected to PCR for potential virulence gene detection. The results of the PCR showed 38/40 strains positive for the cadF, cj1349, ciaB, and tlyA genes, 39/40 strains positive for the pldA gene, and 40/40 strains positive for the mviN gene. None of the strains presented the irgA gene. Hemagglutinin (hecA gene) and hemolysin (hecB) genes were detected in 21/40 and 16/40 strains, respectively. The SE-AFLP showed a great genetic diversity, but some clonally groups were disseminated in various tanks. These data reveal that the strains presented the same virulence traits described from Arcobacter isolated from food-borne disease in humans. |
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