| HPLC-ELSD法测定黄芪精质量标准的研究 |
| |
| 引用本文： | 王雨林,黄新兰.HPLC-ELSD法测定黄芪精质量标准的研究[J].畜牧与饲料科学,2012,33(9):21-21,25. |
| |
| 作者姓名： | 王雨林 黄新兰 |
| |
| 作者单位： | 1. 江苏畜牧兽医职业技术学院,江苏泰州,225300
2. 扬子江药业集团,江苏泰州,225300 |
| |
| 摘 要： | 采用HPLC-ELSD法对黄芪精中黄芪甲苷的含量进行分析。色谱柱为AgilentC18(5μm,4.6mm×250mm),柱温为35℃;流动相:乙腈—水(34:66);流速:1.0mL/min;ELSD检测器参数:漂移管温度为65℃;空气压力为30psi。黄芪甲苷在32.12～321.20μg范围内线性关系良好(r=0.9998),平均回收率为99.26%,RSD=2.75%(n=6)。该法简便、准确、重现性好,可作为黄芪精质量控制方法。
|
| 关 键 词： | 黄芪精 黄芪甲苷 HPLC-ELSD |
Study on Quality Standard of Astragalus Essence Determined by HPLC-ELSD |
| |
| WANG Yu-lin,HUANG Xin-lan.Study on Quality Standard of Astragalus Essence Determined by HPLC-ELSD[J].Animal Husbandry and Feed Science,2012,33(9):21-21,25. |
| |
| Authors: | WANG Yu-lin HUANG Xin-lan |
| |
| Institution: | 1.Jiangsu Animal Husbandry ＆ Veterinary College,Taizhou 225300,China;2.Yangtze River Pharmaceutical Group Co.,Ltd.,Taizhou 225300,China) |
| |
| Abstract: | The content of astragaloside Ⅳ in Astragalus essence was determined by HPLC-ELSD.The chromatography column was Agilent C18（5 μm,4.6 mm×250 mm）;the column temperature was 35 ℃;the mobile phase was acetonitrile and water（34：66）;the flow rate was 1.0 mL/min;the temperature of drift tube of ELSD detector was 65 ℃,and the air pressure was 30 psi.Results showed that the astragaloside Ⅳ within 32.12-321.20 μg had good linear relationship（r=0.999 8）;average recovery was 99.26%,and the RSD was 2.75%（n=6）.It is suggested that the method is simple and accurate and has good reproducibility,so it could be used in quality control method of Astragalus essence. |
| |
| Keywords: | Astragalus essence astragaloside Ⅳ HPLC-ELSD |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
