CRISPR/Cas9基因编辑技术原理及其在牧草中的应用

CRISPR/Cas9系统是一种新型RNA靶向的基因编辑系统。在近几年的研究中, CRISPR/Cas9基因编辑技术的发展十分迅猛, 因其具有经济、高效、简便的特点而广泛应用于育种、医疗、工业等领域。介绍了2种新型基因编辑技术, 阐述了CRISPR/Cas9的结构、功能以及CRISPR/Cas9基因编辑技术原理, 综述了该技术在牧草中的应用研究进展, 以期为利用CRISPR/Cas9系统研究牧草基因功能及改良牧草性状提供思路。

Principle of CRISPR/Cas9 Gene Editing Technology and Its Application in Forage Grass

The CRISPR/Cas9 is a new type of RNA-targeted gene editing system. In recent years, the CRISPR/Cas9 gene editing technology has gained rapid development. Due to its economical, efficient and convenient features, CRISPR/Cas9 gene editing technology is widely used in breeding, medicine, industry and other fields. This paper introduces two types of newly developed gene editing technologies, elaborates the structure and function of CRISPR/Cas9, expounds the principle of CRISPR/Cas9 gene editing technology, and summarizes the application of this technology in forage grass, in hoping to provide references for investigating the forage grass gene function and improving forage grass traits by applying CRISPR/Cas9 gene editing technology.