| 双峰驼肝脏CYP3A亚酶活性的初步研究 |
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| 引用本文： | 王学恩,;裴乐,;格日勒图亚,;哈斯苏荣.双峰驼肝脏CYP3A亚酶活性的初步研究[J].畜牧与饲料科学,2014(6):22-24. |
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| 作者姓名： | 王学恩 ;裴乐 ;格日勒图亚 ;哈斯苏荣 |
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| 作者单位： | [1]阜新高等专科学校农牧系,辽宁阜新123000; [2]内蒙古农业大学兽医学院,内蒙古呼和浩特010018; [3]内蒙古乌审旗兽医局,内蒙古乌审旗017300 |
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| 基金项目： | 国家自然科学基金项目（31260623）. |
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| 摘 要： | CYP3A亚酶参与45%~60%常用药物的生物转化,在机体处置外源性物质的过程中发挥着重要作用。为了探讨双峰驼肝脏CYP3A亚酶活性,采用改进的钙离子沉淀法制备肝微粒悬液,并借助生物化学方法对双峰驼CYP3A亚酶活性进行了初步研究。结果表明,钙离子沉淀法制备的双峰驼肝微粒体蛋白含量为(1.541±0.264)mg/mL,CYP总酶含量为(0.412±0.063)nmol/mg,红霉素-N-脱甲基酶的活性为(1.159±0.001)nmol/(mg·min)。以上研究结果为今后借助探针药物检测双峰驼CYP3A亚酶的体外代谢活性的深入研究奠定了基础。
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| 关 键 词： | 双峰驼 肝微粒体 CYP3A亚酶活性 |
Preliminary Studies on CYP3A Sub-enzyme Activities of Bactrian Camel |
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| Institution: | WANG Xue-en, PEI Le, Geriletuya, Hasisurong (1.Department of Agriculture and Animal Husbandry,Fuxin Higher Training College,Fuxin 123000,China;2.College of Veterinary Medicine, Inner Mongolia Agricultural University,Hohhot 010018 ,China;3.Veterinary Burean of Wushen Banner in Inner Mongolia,Weshen 017300,China) |
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| Abstract: | CYP3A sub-enzymes participate in biotransformation of 45%-60% of commonly used drugs, and play an important role in regulating the interaction of body and external environment and maintaining homeostasis. In order to study the activity of hepatic CYP3A sub-enzymes, the hepatic microsome was prepared by the Ca^2＋ precipitation method, and the activities of CYP3A sub-enzyme was preliminarily studied by using biochemical methods. The result showed that the protein content of hepatic microsome was （1.541±0.264） mg/mL, the content of total CYP was （0.412±0.063 ） nmol/mg, and the activity of erythromycin-N- demethylase was （1.159±0.001） nmol/（mg· min ）, respectively. The results laid a good foundation for further research of metabolic activity of CYP3A subfamily in vitro by using probe drug detection. |
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| Keywords: | bactrian camel liver microsome CYP3A sub-activity |
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