固态发酵葡萄皮渣生产生物饲料的菌种筛选

为了筛选适宜固态发酵葡萄皮渣生产生物饲料的菌种,以葡萄皮渣为主要原料,以真蛋白含量为评价指标,采用产朊假丝酵母、酿酒酵母、嗜酸乳杆菌、植物乳杆菌4株菌的单菌和多菌组合进行固体发酵试验筛选最佳发酵菌种,同时研究了固体培养基灭菌与不灭菌工艺对其产物真蛋白含量的影响。结果表明,葡萄皮渣固体培养基不灭菌发酵产物的真蛋白含量高于灭菌处理;4株菌中产朊假丝酵母单菌发酵后产物的真蛋白含量最高,为13.75%;产朊假丝酵母和嗜酸乳杆菌双菌组合的发酵效果优于三菌和四菌发酵,发酵后产物的真蛋白含量最高,为13.88%。由该试验结果可以确定固态发酵葡萄皮渣的最佳菌种组合为产朊假丝酵母+嗜酸乳杆菌。

Screening of Mixed Strains for Producing Biological Feed by Solid-state Fermentation with Grape Skin Residues

In order to screen the strains suitable for producing biological feed by solid-state fermentation with grape skin residues, the grape skin residues were used as the main raw material, and the true protein content was used as the evaluation index. To screen the optimal strains for fermentation use, the solid-state fermentation experiment was conducted with single stain or multi-strain combinations of Candida utilis, Saccharomyces cerevisiae, Lactobacillus acidophilus and Lactobacillus plantarum. Furthermore, the effect of solid medium sterilization or non-sterilisation process on the true protein content of the fermentation products was assessed. The results showed that the non-sterilized solid medium containing grape skin residues had higher true protein content in the fermentation products. For single strain fermentation experiment, the highest true protein content （13.75%） was observed in the products fermented with Candida utilis. For mixed strains fermentation experiment, the dual-combination of Candida utilis and Lactobacillus acidophilus had the highest true protein content （13.88%） in fermentation products, and showed better fermentation effect compared to the triple- or quadruple-combination of other strains. It was indicated that the best strains combination for the solid-state fermentation of grape skin residues was Candida utilis and Lactobacillus acidophilus.