肉牛屠宰线不同工序中沙门菌和大肠杆菌污染调查及分离鉴定

为了确定肉牛屠宰线沙门菌和大肠杆菌的污染情况,选取山西省2家屠宰厂(A、B),分别在屠宰线7道不同工序和屠宰刀具上进行采样,采用传统方法进行沙门菌和大肠杆菌的分离、培养,利用生化鉴定法和16SrDNA序列分析法对分离菌株进行鉴定。结果表明:A厂所有工序均未发现沙门菌和大肠杆菌污染情况;B厂所有工序均未检测出沙门菌,但在宰前皮毛和粪便中检测到8株疑似大肠杆菌,经生化鉴定法和16SrDNA序列分析法全部确定为大肠杆菌。综上表明,选取的2个肉牛屠宰加工企业屠宰线相关工序消毒、减菌措施到位,但应注重宰前动物的规范化管理,以减少宰前污染。

Investigation on Contamination of Salmonella and Escherichia coli in Different Processes of Beef Cattle Slaughter Line

In order to determine the contamination of Salmonella and Escherichia coli(E.coli)in beef cattle slaughter line,two slaughterhouses(A and B)in Shanxi Province were selected for sampling from seven different processes and butcher steels.The Salmonella and E.coli strains were isolated and cultured by using traditional methods.The isolates were subsequently identified with biochemical identification method and 16S rDNA sequence assay method.The results showed that no contamination caused by Salmonella and E.coli were observed in all tested processes and butcher steels in slaughterhouse A;while all of the tested processes in slaughterhouse B were negative for contamination caused by Salmonella,eight phenotypically and molecularly identified E.coli isolates were found in fur and feces before slaughter.The combined data suggest that although the disinfection and reduction of bacteria associated with contamination of beef cattle slaughter line in the two selected slaughtering and processing enterprises are generally qualified,the standardized management of pre-slaughter animals should be paid attention to reduce pre-slaughter pollution.