黑龙江省大庆市部分地区牛轮状病毒腹泻的分子流行病学调查

为了对黑龙江省大庆市部分地区犊牛轮状病毒腹泻的流行情况进行调查,应用RT-PCR技术对随机采取的6份犊牛腹泻粪便样品的轮状病毒VP7基因进行扩增,采用多重半套式PCR方法对VP7基因阳性样本进行分型鉴定。结果显示,6份犊牛腹泻粪便样品中牛轮状病毒VP7基因均为阳性;VP7基因阳性样本经RT-PCR分型鉴定,均属于G10型。该研究结果表明大庆地区引起犊牛轮状病毒腹泻的轮状病毒主要为G型,因此需要针对G型轮状病毒加以防控。

Molecular Epidemiology of Bovine Rotavirus Infections in Some Areas of Daqing City of Heilongjiang Province

In order to investigate the prevalence of rotavirus infection associated calf diarrhea in some areas of Daqing City of Heilongjiang Province, six randomly collected fecal samples from calf with diarrhea were detected for the presence of VP7 gene of bovine rotavirus by suing RT-PCR assay, and the VP7 gene positive samples were subjected to genotyping via multiple semi-nested PCR assay. The results showed that all of the six clinical samples were positive for VP7 gene of bovine rotavirus, and they were all proved to belong to G10 type. It was indicated that the G-type bovine rotavirus was one of the main causative agents associated with the calf diarrhea in Daqing area. Therefore, the prevent and control measures targeting on G-type bovine rotavirus infection should be implemented.