甘薯膳食纤维提取工艺的研究

试验模拟甘薯淀粉提取过程获得甘薯渣,通过单因素与正交试验研究了各种酶添加量对膳食纤维提取率的影响,确定糖化酶为主要酶,并且通过正交试验确定了糖化酶酶解条件(温度、时间、pH值)的最优组合。试验结果表明,各种酶的最适添加量分别为:α-淀粉酶1.4mL/g、胰蛋白酶0.5mL/g、糖化酶为5.0mL/g,其中,糖化酶是影响提取率的主要酶。糖化酶酶解的最佳工艺条件为:温度60℃,时间40min,pH值4.5。在最优工艺条件下制备甘薯渣膳食纤维产品,对产品进行分析表明,总膳食纤维提取率为81.6%,其中可溶性膳食纤维提取率可达25.7%,甘薯渣膳食纤维的膨胀力和持水力分别达到3.42mL/g和665%。

Study on the Extraction Technology of Sweet Potato Dietary Fiber

The sweet potato residue was got by simulating the extraction process of sweet potato starch.Effects of the addition amounts of various enzymes on the extraction rate of dietary fiber were studied by single-factor and orthogonal experiments,and the enzymatic hydrolysis conditions of glucoamylase were optimized by orthogonal experiment.Results showed that the optimal addition amounts of various enzymes were α-amylase 1.4 mL/g,trypsin 0.5 mL/g and glucoamylase 5.0 mL/g,and the glucoamylase was identified as the main enzyme.The optimal conditions for glucoamylase hydrolysis were 60 ℃,40 min and pH 4.5.The extraction rates of total dietary fiber and soluble dietary fiber under the optimal conditions were 81.6% and 25.7%,respectively.The expansion and the water holding capacity of dietary fiber reached 3.42 mL/g and 665%,respectively.