滩羊12个微卫星基因座遗传多态性研究

滩羊是我国优良的地方绵羊品种,开展滩羊微卫星标记和体重性状之间的相关性研究有利于建立基于分子标记的新育种体系。利用与绵羊体重主效基因高度连锁的12个微卫星标记(BL1080、BM6506、BM7145、BMS2263、BMS678、ILSTS004、ILSTS30、MCM58、BMS1591、BMS1636、CSSM004、MCM130),对51只滩羊进行了遗传检测。应用琼脂糖凝胶电泳检测微卫星的PCR扩增产物,计算12个微卫星基因座的等位基因频率、多态信息含量、基因纯合度和杂合度。结果表明,所选择的12个微卫星位点均为高度多态基因座,其中,BMS1636的遗传变异最大,BL1080的遗传变异最小。研究结果为滩羊种质特性研究提供了基础数据。

Genetic Polymorphisms of Twelve Microsatellite Loci in Tan Sheep

Tan sheep is an excellent local sheep breed in China.Investigating the correlation between microsatellite marker and body weight trait is conducive to establish the molecular marker-based novel breeding system for Tan sheep.In this study,the polymorphisms of 12 microsatellite loci,including BL1080,BM6506,BM7145,BMS2263,BMS678,ILSTS004,ILSTS30,MCM58,BMS1591,BMS1636,CSSM004 and MCM130 which were closely linked to the major genes associated with weight trait in sheep,were detected in 51 ewes of Tan sheep.The PCR amplified products of the microsatellite loci were detected by agarose gel electrophoresis.The allele frequency,polymorphism information content,gene homogeneity and heterozygosity of the 12 microsatellite loci were calculated.The results demonstrated that the 12 selected microsatellite markers were all highly polymorphic loci;the microsatellite locus BMS1636 had the highest polymorphism among the 12 tested microsatellite loci,while the microsatellite locus BL1080 showed the lowest polymorphism.These results provide basic molecular data for the research on the germplasm characteristics of Tan sheep.