ZD制剂对RAW264.7细胞分泌TNF-α水平及TNF-α诱导L929细胞死亡的影响

目的]研究ZD制剂对炎症模型细胞中TNF-α含量及对TNF-α诱导小鼠成纤维细胞L929死亡的影响。方法]通过脂多糖（LPS）刺激小鼠腹腔巨噬细胞RAW264.7细胞系来构建炎症细胞模型,使用5种不同浓度（100、10、1、0.1、0.01μg/m L）的ZD制剂进行试验,并用ELISA法检测ZD制剂干预前后细胞上清液中TNF-α浓度。通过TNF-α刺激小鼠成纤维细胞L929细胞系来构建细胞毒性模型,采用MTT法检测ZD制剂干预前后L929细胞活力。结果]ZD制剂浓度为100、10、1、0.1μg/m L时,TNF-α浓度与LPS模型组相比,差异极显著（P〈0.01）;ZD制剂浓度为0.01μg/m L时,TNF-α浓度与LPS模型组相比,差异显著（P〈0.05）。经100、10μg/m L ZD制剂干预后的小鼠成纤维细胞L929细胞活力与TNF-α组相比显著提高。结论]ZD制剂可以通过降低TNF-α的分泌量和降低TNF-α的生物学活性来达到抗炎的目的。

Effects of ZD Preparation on Secretion of TNF-α by RAW264.7 Cells and TNF-αInduced L929 Cells Death

Objective]To study the effects of ZD preparation on the concentration of TNF-α in a cellular model of inflammation and the death of mouse fibroblast L929 cells induced by TNF-α. Methods] The mouse peritoneal macrophage RAW264.7 cell line was stimulated by lipopolysaccharide（LPS） to establish a cellular model of inflammation, and five different concentrations of ZD preparation（100, 10, 1, 0.1, 0.01 μg/m L） were included in this study. ELISA method was used to detect the TNF-αconcentration in the cell supernatant before and after ZD preparation intervention. The cytotoxic model was established with mouse fibroblast L929 cell line by stimulation of TNF-α, and the activity of L929 cells was determined by MTT method before and after the intervention of ZD preparation. Results] The 100, 10, 1 and 0.1 μg/m L of ZD preparation groups had extremely significant difference in TNF-α concentration compared to the LPS model group（P〈0.01）, and the 0.01 μg/m L of ZD preparation group showed significant difference in TNF-α concentration compared to the LPS model group（P〈0.05）. In addition, the activity of mouse fibroblast L929 cells were significantly elevated after the intervention of 100 and 10 μg/m L of ZD preparation compared to the TNF-α group. Conclusion] ZD preparation exerts the anti-inflammatory effects via reducing the secretion of TNF-α and inhibiting the biological activity of TNF-α.