体外法研究不同剂量延胡索酸二钠对绵羊瘤胃发酵的影响

利用体外产气法研究添加不同剂量延胡索酸二钠对绵羊瘤胃发酵的影响。采用单因素完全随机设计,发酵底物以可溶性淀粉和纤维素为碳源,以胰蛋白胨为氮源,对照组不添加延胡索酸二钠,试验1、2、3组分别添加占发酵底物干物质1.0%、2.0%和3.0%的延胡索酸二钠。结果显示,与对照组相比,添加1%的延胡索酸二钠（试验1组）可显著（P<0.05）提高瘤胃的总挥发性脂肪酸、乙酸、丙酸、丁酸产量以及体外产气量和干物质降解率;添加2%的延胡索酸二钠（试验2组）可显著（P<0.05）提高瘤胃的总挥发性脂肪酸、乙酸、丙酸、丁酸产量和干物质降解率;添加3%的延胡索酸二钠组（试验3组）的瘤胃氨氮浓度、总挥发性脂肪酸和乙酸产量、产气量、干物质降解率与对照组差异不显著（P>0.05）,但丙酸产量显著增加（P<0.05）。综上表明,添加1%和2%的延胡索酸二钠会显著提高绵羊瘤胃发酵性能,但添加3%的延胡索酸二钠,瘤胃发酵能力有所降低。

Effect of Different Levels of Disodium Fumarate on Rumen Fermentation of Sheep InVitro

In this study, in vitro gas production technique was used to investigate the effect of different levels of disodium fumarate on rumen fermentation in sheep with a one-factor completely randomized design. The soluble starch and cellulose were used as carbon sources, tryptone was used as nitrogen source. Disodium fumarate accounting for 1.0%, 2.0% and 3.0% of the dry matter of fermentation substrate were added in experimental groups 1, 2 and 3, respectively. The control group received no addition of disodium fumarate. The results showed that compared with the control group, adding 1% of disodium fumarate （experimental group 1） could significantly （P<0.05） improve the output of total volatile fatty acids, acetic acid, propionic acid and butyric acid, in vitro gas production, and dry matter degradation rate; the significantly （P<0.05） increased output of total volatile fatty acids, acetic acid, propionic acid and butyric acid as well as significantly （P<0.05） elevated dry matter degradation rate were observed in experimental group 2 compared to the control group; there was no significant （P>0.05） differences in ammonia nitrogen concentration, total volatile fatty acid and acetic acid output, gas production and dry matter degradation rate between experimental group 3 and control group, but experimental group 3 had significantly （P<0.05） increased propionic acid production. In conclusion, adding 1% or 2% fumarate disodium can significantly improve the rumen fermentation performance of sheep, but adding 3% fumarate disodium exhibited an inhibiting effect on rumen fermentation capacity to a certain extent.