Elevated levels of fragmented laminin-5 gamma2-chain in bronchoalveolar lavage fluid from dogs with pulmonary eosinophilia |
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| Authors: | Rajamäki Minna M Järvinen Anna-Kaisa Sorsa Timo A Tervahartiala Taina I Maisi Päivi S |
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| Institution: | 1. Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, 00014 University of Helsinki, P.O. Box 57, Hämeentie 57, Helsinki, Finland;2. Department of Oral and Maxillofacial Diseases, Helsinki University Central Hospital (HUCH), Institute of Dentistry, 00014 University of Helsinki, Orton Research Institute and Orthopedic Hospital of Invalid Foundation, Helsinki, Finland;1. Division of Nephrology, University of Washington School of Medicine, 750 Republican Street, Seattle, WA 98109, USA;2. Department of Medicine, Division of Nephrology, The Icahn School of Medicine at Mount Sinai, 1468 Madison Avenue, New York, NY 10029, USA;3. Department of Pathology, University of Chicago, 5841 S Maryland Ave, Chicago, IL 60637, USA;4. Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Elm & Carlton Streets, Buffalo, NY 14263, USA;5. The Roslin Institute, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK;1. Departments of Pathology and Microbiology and Immunology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305, USA;2. Division of Bioorganic Chemistry, Faculty of Chemistry, Wroclaw University of Technology, Wyb. Wyspianskiego 27, 50-370 Wroclaw, Poland |
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| Abstract: | Inflammation causes epithelial cell sloughing and basement membrane (BM) exposure in canine pulmonary eosinophilia (PE), leading to degradation of the epithelial cell attachment component, laminin-5 gamma2-chain, into small molecular weight fragments. The subsidence of inflammation after treatment down-regulates degradation. Laminin-5 gamma2-chain levels and molecular forms in bronchoalveolar lavage fluid (BALF) were analysed semiquantitatively by Western immunoblotting to compare PE affected (n=20) and healthy dogs (n=16) as well as PE dogs (n=6) before and after corticosteroid treatment. PE dogs expressed significantly elevated levels of total (P<0.01), 36 kDa (P<0.05) and 53 kDa (P<0.05) laminin-5 gamma2-fragments. The 36 Da fragment decreased significantly (P<0.05) after treatment. The laminin-5 gamma2-chain degradation products may be linked to epithelial cell sloughing and BM exposure or healing. |
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| Keywords: | Eosinophil Lung inflammation Laminin-5 γ 2-chain Basement membrane Destruction |
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