Random Amplification of Polymorphic DNA Fingerprinting of Trypanosoma evansi |
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| Authors: | Basagoudanavar SH Rao JR Singh RK Butchaiah G |
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| Institution: | (1) National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar, 243 122, India;(2) Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, 243 122, India |
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| Abstract: | The total genomic DNAs from Trypanosoma evansi isolates of bubaline, equine and cameline origin were amplified by polymerase chain reaction using eight arbitrarily selected 10-base primers. Informative band patterns were obtained for all isolates analysed. Depending upon the T. evansi isolate–primer combination, between 1 and 11 reproducible DNA fingerprints of 205 to 3016 bp were amplified, suggesting minor and major differences in their RAPD (random amplified polymorphic DNA) profiles. |
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| Keywords: | buffalo camel DNA haemoprotozoa horse identification PCR polymerase chain reaction Trypanosoma evansi |
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