The efficacy of FMD vaccine reduced non-structural proteins with a mAb against 3B protein |
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Authors: | Dong Li Zai-Xin Liu Pu Sun Yong-Liang Li Zeng-Jun Lu Mei-Na Tian Ying-Li Chen Bao-Xia Xie Hui-Fang Bao Yuan-Fang Fu Yi-Mei Cao Ping-Hua Li Xin-Wen Bai Jia-Chuan Sun Jian-Hong Guo Xiang-Tao Liu Qing-Ge Xie |
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Institution: | 1. Key Laboratory of Animal Virology of the Ministry of Agriculture, National Foot-and-Mouth Disease Reference Laboratory of China, State Key Laboratory of Veterinary Etiologic Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China, 730046
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Abstract: | A monoclonal antibody, 3BIgG, against the prokaryotically expressed foot-and-mouth disease virus (FMDV) non-structural protein (NSP) 3B was obtained. The 3BIgG-sepharose conjugant (3BmAb-6BFF) was prepared by adding the purified 3BIgG into epoxy-activated sepharose 6BFF, incubating with the inactivated FMDV, and then removing the sepharose by centrifugation. The vaccine was made from the supernatant emulsified with oil-adjuvant ISA206. Ten guinea pigs, 26 pigs and six cattle were vaccinated, and a vaccination control group was included without treatment with 3BmAb-6BFF. After 28 days, 9/10 pigs challenged with FMDV were protected, this result was the same as the control group, indicating that the vaccine potency was not reduced after treatment with 3BmAb-6BFF. The other animals were vaccinated weekly for nine weeks, and serum samples were collected to detect 3ABC-antibody titers. The results showed that 3ABC-antibody production was delayed and the positive antibody rates were lower when vaccination was carried out using vaccines treated with 3BmAb-6BFF compared with untreated vaccines. The findings of this study suggest that it is possible to reduce NSPs using a mAb-sepharose conjugant in FMD vaccines without reducing their efficacy. |
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