| 基于基因探针扫描技术高通量检测乳制品中赭曲霉毒素A产生菌 |
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| 引用本文： | 孔文平,李裕,郭锦材,李灿,朱湘成,陈同强.基于基因探针扫描技术高通量检测乳制品中赭曲霉毒素A产生菌[J].乳业科学与技术,2022,45(1):20-25. |
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| 作者姓名： | 孔文平 李裕 郭锦材 李灿 朱湘成 陈同强 |
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| 作者单位： | 1.中南大学湘雅国际转化医学联合研究院，湖南 长沙 410083；2.长沙市口腔医院，湖南 长沙 410006；3.食品安全监测与预警湖南省重点实验室，湖南 长沙 410111；4.湖南省食品质量监督检验研究院，湖南 长沙 410000；5.组合生物合成与天然产物药物湖南省工程研究中心，湖南 长沙 410011 |
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| 基金项目： | 食品安全监测与预警湖南省重点实验室开放基金课题项目(2020KFJJ13);湖南省市场监督管理局科技计划项目(2020KJJH03);长沙市自然科学基金项目(B2020277);食品安全快速检测与溯源关键技术研究与示范项目(2020SK2128)。 |
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| 摘 要： | 为快速、准确地检测出乳制品中赭曲霉毒素A(ochratoxin A,OTA)产生菌,以便评估乳制品中OTA污染的潜在风险,根据已阐明的O TA生物合成途径,选择与O TA产生有关的卤化酶基因区域设计兼并引物,建立针对OTA产生菌的聚合酶链式反应(polymerase chain reaction,PCR)检测方法.对1...
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| 关 键 词： | 基因探针 高通量 产毒真菌 赭曲霉毒素A 乳制品 风险评估 |
High-Throughput Detection of Ochratoxin A-Producing Fungi in Dairy Products Using Gene Probe Scanning Technology |
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| KONG Wenping,LI Yu,GUO Jincai,LI Can,ZHU Xiangcheng,CHEN Tongqiang.High-Throughput Detection of Ochratoxin A-Producing Fungi in Dairy Products Using Gene Probe Scanning Technology[J].JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY,2022,45(1):20-25. |
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| Authors: | KONG Wenping LI Yu GUO Jincai LI Can ZHU Xiangcheng CHEN Tongqiang |
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| Institution: | 1.Xiangya International Institute of Translational Medicine, Central South University, Changsha 410083, China; 2.Changsha Stomatological Hospital, Changsha 410006, China; 3.Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning, Changsha 410111, China; 4.Hunan Institute of Food Quality Supervision Inspection and Research, Changsha 410000, China; 5.Hunan Engineering Research Center of Combinatorial Biosynthesis and Natural Products Medicine, Changsha 410011, China |
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| Abstract: | To quickly and accurately evaluate the risk of ochratoxin A (OTA) pollution in dairy products, a method to detect fungi capable of producing OTA in dairy products was developed using gene probe scanning technology. According to the elucidated biosynthesis pathway of OTA, the halogenase gene related to OTA production was selected to design degenerate primers to establish a polymerase chain reaction (PCR) method for the detection of OTA-producing fungi. One OTAproducing strain (Aspergillus ochraceus) and five strains not able to produce OTA (Aspergillus cristatus, Aspergillus flavus, Fusarium moniliforme, Issatchenkia terricola, and Saccharomyces cerevisiae) were selected to test the PCR method. It was found that DNA from the OTA-producing strain but not the non-OTA producers could be amplified by PCR. Moreover, this method had high accuracy and sensitivity for detecting polluted dairy products. |
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| Keywords: | gene probe high-throughput mycotoxin-producing fungi ochratoxin A dairy products risk assessment |
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