| 含乳特殊医学用途配方食品中沙门氏菌检测即用型质控样品研制 |
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| 引用本文： | 张涛,张瑞,张雅伦,许苗苗,王慧,陈晨,周巍,史国华.含乳特殊医学用途配方食品中沙门氏菌检测即用型质控样品研制[J].乳业科学与技术,2021,44(4):29-33. |
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| 作者姓名： | 张涛 张瑞 张雅伦 许苗苗 王慧 陈晨 周巍 史国华 |
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| 作者单位： | （河北省食品检验研究院，河北省食品安全重点实验室，河北 石家庄 050200） |
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| 基金项目： | 科技部“食品安全关键技术研发”重点专项（2017YFC1601400）；国家市场监督管理总局技术保障专项（2020YJ006） |
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| 摘 要： | 为评估即用型沙门氏菌质控样品在基质辅助激光解析电离飞行时间质谱技术鉴定含乳特殊医学用途配方食品中沙门氏菌中的应用价值，通过验证即用型沙门氏菌质控样品的均匀性、稳定性，对－20 ℃贮藏0、14、28 d沙门氏菌质控样品进行质谱鉴定，最后用VITEK全自动微生物分析系统进行生化鉴定。结果表明：即用型沙门氏菌质控样品稳定性良好、均匀性符合要求，所得质谱图相近，VITEK生化鉴定结果为沙门氏菌；即用型沙门氏菌质控菌株可以作为含乳特殊医学用途配方食品中沙门氏菌检测质控样品使用。
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| 关 键 词： | 沙门氏菌 即用型质控样品 含乳特殊医学用途配方食品 稳定性 基质辅助激光解析电离飞行时间质谱 |
Development of Ready-to-Use Quality Control Samples for the Detection of Salmonella in Formula Foods for Special Medical Purposes Containing Milk |
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| ZHANG Tao,ZHANG Rui,ZHANG Yalun,XU Miaomiao,WANG Hui,CHEN Chen,ZHOU Wei,SHI Guohua.Development of Ready-to-Use Quality Control Samples for the Detection of Salmonella in Formula Foods for Special Medical Purposes Containing Milk[J].JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY,2021,44(4):29-33. |
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| Authors: | ZHANG Tao ZHANG Rui ZHANG Yalun XU Miaomiao WANG Hui CHEN Chen ZHOU Wei SHI Guohua |
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| Institution: | (1.Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang 050200, China; 2.College of Life Science, Hebei Normal University, Shijiazhuang 050000, China) |
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| Abstract: | In order to evaluate the application value of ready-to-use Salmonella quality control samples in matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) identification of Salmonella in milk-containing formula foods for special medical purposes, this experiment evaluated the uniformity and stability of the quality control samples. After being stored at 20 ℃ for 0, 14 and 28 days, these samples were identified by mass spectrometry and the VITEK automatic microbial analysis system. The results showed that the quality control samples had good stability and uniformity, and their mass spectra were similar to each other. The VITEK system confirmed their identity as Salmonella. In conclusion, the ready-to-use Salmonella quality control strains can be used for the detection of Salmonella in milk-containing formula foods for special medical purposes. |
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| Keywords: | Salmonella ready-to-use quality control samples milk-containing formula foods for special medical purposes stability matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
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