| T-RFLP技术及其在动物胃肠道微生物群落多样性研究中的应用 |
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| 引用本文： | 于萍,王加启,卜登攀,邓露芳,刘开朗.T-RFLP技术及其在动物胃肠道微生物群落多样性研究中的应用[J].乳业科学与技术,2008,31(6). |
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| 作者姓名： | 于萍 王加启 卜登攀 邓露芳 刘开朗 |
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| 作者单位： | 中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,北京,100193 |
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| 摘 要： | 随着分子生物学技术的飞速发展,越来越多的新兴研究手段应用于胃肠道微生态系统的研究之中。介绍了末端限制性片段长度多态性技术(T-RFLP)的基本原理、操作流程和优缺点,并总结了该技术在动物胃肠道微生态研究中的应用现状。
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| 关 键 词： | 末端限制性片段长度多态性 微生物多样性 胃肠道微生态 |
T-RFLP and its Application in Gastrointestinal Microbial Diversity |
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| Yu Ping,Wang Jiaqi,Bu Dengpan,Deng Lufang,Liu Kailang.T-RFLP and its Application in Gastrointestinal Microbial Diversity[J].JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY,2008,31(6). |
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| Authors: | Yu Ping Wang Jiaqi Bu Dengpan Deng Lufang Liu Kailang |
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| Abstract: | With the rapid development of the molecular biotechnologies,more and more emerging approaches are applied in the investigation of microbial ecology of gastrointestinal tract. The present review represents a comprehensive summary of the terminal restriction polymorphism (T-RFLP) technology,covering aspects including its basic principles,operational procedures,advantages and disadvantages and its applications in animal gastrointestinal microbial ecology. |
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| Keywords: | terminal restriction fragment length polymorphism ( T -RFLP) microbial diversity gastrointestinal microbial ecology |
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