| 高效液相色谱法测定乳品中乳铁蛋白的含量 |
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| 引用本文： | 岳虹,刘春霞,吕海燕,李素琴,赵贞,李翠枝.高效液相色谱法测定乳品中乳铁蛋白的含量[J].乳业科学与技术,2014(4):18-21. |
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| 作者姓名： | 岳虹 刘春霞 吕海燕 李素琴 赵贞 李翠枝 |
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| 作者单位： | 内蒙古伊利实业集团股份有限公司,呼和浩特010010 |
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| 基金项目： | “十二五”国家科技支撑计划项目（2012BAKl7800） |
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| 摘 要： | 建立乳品中乳铁蛋白高效液相色谱的测定方法。采用pH值为7.0磷酸盐缓冲溶液做为提取剂,C_(18)色谱柱分离,不同浓度的乙腈-0.5 mol/L的氯化钠溶液-三氟乙酸的二元系统进行梯度洗脱。每100g奶粉(或每100mL液态奶)中乳铁蛋白添加量为5~10 mg时,回收率大于90%。定量限为0.5 mg/100g。该方法具有结果准确,操作简便,重复性好等优点,可用于乳品中乳铁蛋白的检测。
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| 关 键 词： | 高效液相色谱法 乳铁蛋白 肝素免疫亲和柱 |
High Performance Liquid Chromatography Determination of Lactoferrin in Dairy Products |
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| YUE Hong,LIU Chun-xia,LV Hai-yan,LI Su-qin,ZHAO Zhen,LI Cui-zhi.High Performance Liquid Chromatography Determination of Lactoferrin in Dairy Products[J].JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY,2014(4):18-21. |
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| Authors: | YUE Hong LIU Chun-xia LV Hai-yan LI Su-qin ZHAO Zhen LI Cui-zhi |
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| Institution: | (Inner Mongolia Yili Industrial Group Co. Ltd., Hohhot 010010, China) |
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| Abstract: | A method for the determination of lactoferrin in dairy products by high performance liquid chromatography （HPLC） was developed. Lactoferrin was extracted by the solution of pH 7.0 0.05 mol/L phosphate. The method was performed on C18 column, the analysis was performed by gradient elution using solvent systems acetonitrile-0.5mol/L sodium chloride-TFA. Lactoferrin content was in the range of 5-10 mg/100 g, the recovery was higher than 90%. The limit was 0.5 mg/100 g. The results indicated that this method is accurate, simple and reproducible, and thus suitable for the analysis of lactoferrin in milk products. |
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| Keywords: | high performance liquid chromatography lactoferrin HiTrap Heparin HP column |
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