Arthrographis kalrae soluble antigens present hemolytic and cytotoxic activities |
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| Institution: | 1. State University of Londrina, Rod. Celso Garcia Cid, km 380, 86057-970 Londrina, PR, Brazil;2. University of the Ryukyus, Okinawa, Japan;3. Murata Animal Hospital, Mobara, Chiba, Japan;1. Division of Infectious Disease, Department of Internal Medicine, Tungs’ Taichung MetroHarbor Hospital, Taichung, Taiwan;2. Graduate Institute of Microbiology and Public Health, National Chung Hsing University, Taichung, Taiwan;3. Infection Control Office, Tungs’ Taichung MetroHarbor Hospital, Taichung, Taiwan;4. Department of Medical Research, Tungs’ Taichung MetroHarbor Hospital, Taichung, Taiwan;1. Department of Small Animal, Universidade Federal de Santa Maria, Santa Maria, Brazil;2. Department of Animal Science, Universidade do Estado de Santa Catarina, Chapecó, Brazil;3. Univeridade Luterana do Brasil, Santa Maria, Brazil;4. Department of Clinical and Toxicological Analysis, Universidade Federal de Santa Maria, Brazil;5. Graduate Program, Universidade do Estado de Santa Catarina, Lages, Brazil;6. Department of Large Animal, Universidade Federal de Santa Maria, Santa Maria, Brazil |
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| Abstract: | Arthrographis kalrae is a dimorphic, cosmopolitan and neurotropic fungus that has been described as a rare human pathogen. This study investigated the hemolytic and cytotoxic activities of A. kalrae cell-free antigens (CFAs). Total CFAs and their Sephadex chromatography fractions were tested on mouse erythrocytes for hemolysis and on the P3U1 cell line for cytotoxicity. Hemolytic and cytotoxic activities were detected in distinct molecular mass (MM) fractions. Additionally, antibodies against isogenic erythrocytes sensitized with CFAs (anti-E-CFAs) inhibited hemolysis but not cytotoxicity. Hemolysis was not affected by heating, and a higher reactivity was detected in the carbohydrate-rich fractions, which decreased after reduction by periodate treatment. The pioneering nature of this work is due to the demonstration of the cytotoxic activity in A. kalrae and the suggestion that this activity may be due to molecules distinct from the hemolytic factor, with the latter potentially being a component with a high MM. |
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| Keywords: | Hemolysis Cytotoxicity Fungus Chromatography Antibodies |
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