Association of TNF-α, IL-10 and IL-6 promoter polymorphisms in pulmonary tuberculosis patients and their household contacts of younger age group |
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| Institution: | 1. Bhagwan Mahavir Medical hospital and Research Centre, Hyderabad, Andhra Pradesh, India;2. Department of Transplant Biology& Stem cell, Global Hospital Hyderabad, Andhra Pradesh, India;3. Department of Genetics, Osmania University, Hyderabad, Andhra Pradesh, India;1. Inmunología y Genética Aplicada, S.A. (INGENASA), C/Hermanos García Noblejas 39, 28037, Madrid, Spain;2. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria – Centro de Investigación en Sanidad Animal (INIA-CISA), Ctra Algete-El Casar s/n, 208130, Valdeolmos, Madrid, Spain;3. Instituto de Investigación en Recursos Cinegéticos (CSIC-UCLM), Ronda de Toledo 12, 13005 Ciudad Real, Spain;4. Estación Biológica de Doñana CSIC, Calle Américo Vespucio, s/n, 41092, Seville, Spain;5. Centro de Investigación Biomédica en Red de Epidemiologia y Salud Pública (CIBERESP), Spain;1. Toxicology-Microbiology and Environmental Health Laboratory, Faculty of Sciences, University of Sfax, Tunisia;2. Veterinary Research center of Sfax, Sfax, Tunisia;3. Equipe Microbiologie de l’?uf et des Ovoproduits (MICOV), Agrocampus Ouest, INRA, (UMR1253) Science et Technologie du Lait et de l’?uf, Rennes, France;1. Departament of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina, PR, 86057-970, Brazil;2. Laboratory of Animal Health, Instituto Federal do Maranhão, São Luís, MA, 65095-460, Brazil;3. Departament of Veterinary Medicine, Universidade Federal do Paraná, Curitiba, PR, 80035-050, Brazil;1. CZ Veterinaria S.A, Porriño, Pontevedra, Spain;2. VISAVET Health Surveillance Centre, Complutense University of Madrid, Madrid, Spain;3. Dpto. de Anatomía y Anatomía Patológica Comparadas, Universidad de Córdoba, AgrifoodExcellence International Campus (ceiA3), Córdoba, Spain;4. Infectious Diseases Unit. Instituto Maimonides de Investigación Biomédica de Córdoba (IMIBIC). Hospital Universitario Reina Sofía de Córdoba. Universidad de Córdoba. Cordoba, Spain;5. Dpto. de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain;6. Laboratorios SYVA S.A.U., Technological Park, León, Spain;7. NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario, Animal Health Department, Derio, Bizkaia, Spain;8. MAEVA SERVET S.L., Madrid, Spain;9. Dpto. de Medicina y Cirugía Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain;10. Servicio de Inmunología Microbiana, Centro Nacional de Microbiología, Instituto de Investigación Carlos III, Majadahonda, Madrid, Spain;11. SaBio (Health and Biotechnology), Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM), Ciudad Real, Spain;1. Department of Immunology, Bhagwan Mahavir Medical Research Center, Hyderabad, India;2. Department of Immunology and Molecular Biology, LEPRA Society, Hyderabad, India;3. Department of Genetics, Osmania University, Hyderabad, India |
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| Abstract: | Single nucleotide polymorphisms (SNPs) of cytokine genes have been found to be involved in the clinical outcome of Tuberculosis. The present study was aimed to identify the high risk genotypes in Tuberculosis patients and their household contacts. A total of 490 subjects were studied which includes 150 active pulmonary tuberculosis patients (APTB), 190 household contacts (HHC) and 150 healthy controls (HC). The SNPs of TNF-α (-308A/G), IL-10(-1082G/A) and IL-6(-174G/C) were performed by ARMs PCR. The IL-10 GA genotype showed significant association in APTB and HHC and was 2.3 times higher risk in APTB and 3.7 times in HHC compared to HCs. The A allele was found to be significantly associated with the risk of disease. The CC genotype of IL-6 was found to be significantly associated in APTB and an insignificant positive association in HHCs. The multifactor dimensionality reduction (MDR) analysis indicated that the genotypes of IL-6 were showing high risk with GA genotype of IL-10. In conclusion the gene interaction may be useful for identification of genotypes as biomarkers to distinguish high risk individuals. |
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| Keywords: | Household contacts Single nucleotide polymorphisms MDR TNF-α IL-10 IL-6 |
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