Phenotype evaluation of human and canine isolates of Leishmania infantum |
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| Institution: | 1. Department of Animal Biology, Biology Institute, State Campinas University, Campinas, São Paulo, Brazil;2. Department of Genetics and Evolution, Microbiology and Immunology, Biology Institute, State Campinas University, Campinas, São Paulo, Brazil;3. Superintendência de Controle de Endemias, Seção Técnica de Pesquisa, Controle deVetores, Campinas, São Paulo, Brazil;4. Department of Clinical Pathology, Faculty of Medical Sciences, University of Campinas, São Paulo, Brazil;1. Department of Veterinary Pathology and Public Health, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, 22110, Jordan;2. Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, 22110, Jordan;3. School of Medicine, Jordan University of Science and Technology, Irbid, 22110, Jordan;4. Department of Global Health, Milken Institute School of Public Health, George Washington University, Washington D.C, USA;5. Department of Global and Community Health, College of Health and Human Services, George Mason University, Fairfax, VA, USA;1. Department of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt;2. Department of Virology, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt;3. Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt;1. Centro de Pesquisa em Saúde Animal, Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Eldorado do Sul, RS, Brazil;2. Área de Vida Assessoria e Consultoria em Biologia e Meio Ambiente, Canoas, RS, Brazil;3. Universidade Luterana do Brasil, Canoas, RS, Brazil;4. Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Jaboticabal, SP, Brazil;5. Museu de Ciências Naturais (MCN), Fundação Zoobotânica do Rio Grande do Sul (FZB-RS), Porto Alegre, RS, Brazil;1. Department of Veterinary Physiology and Biochemistry, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, 125004, Haryana, India;2. Department of Bio-technology, GLA University, Post-Chaumuhan, Dist., Mathura, Uttar Pradesh, 281 406, India;3. Department of Veterinary Clinical Complex, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, 125004, Haryana, India;1. Mestre em Ciência Animal, Ilhéus, BA, Brazil;2. Doutoranda do Programa de Pós-Graduação em Ciência Animal da Universidade Estadual de Santa Cruz—UESC, Ilhéus, BA, Brazil;3. Médica Veterinária, Ilhéus, BA, Brazil;4. Doutorando em Patologia Humana e Experimental no Centro de Pesquisas Gonçalo Moniz (FIOCRUZ-BA), Salvador, BA, Brazil;5. Doutorando do Programa de Pós—Graduação em Genética e Biologia Molecular da Universidade Estadual de Santa Cruz—UESC, Ilhéus, BA, Brazil;6. Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz—UESC—Campus Soane Nazaré de Andrade, Hospital Veterinário, km 16 Rodovia Jorge Amado, CEP 45662-900 Ilhéus, BA, Brazil;1. Division of Epidemiology, ICAR- Indian Veterinary Research Institute, Bareilly, India;2. Veterinary Clinical Complex, Veterinary College, Gadag, Karnataka, India;3. Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Mizoram, India;4. Division of Veterinary Public Health, ICAR- Indian Veterinary Research Institute, Bareilly, India |
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| Abstract: | Human visceral leishmaniasis (VL) and canine leishmaniasis (CanL) in countries of South and Central America are caused by Leishmania infantum and has been endemic in Brazil for several years. The parasite biodiversity as well as the pharmacologic properties of drugs and the host species, are involved in the efficacy or inefficacy of leishmaniasis treatments. Although there are substantial number of reports describing the genetic characterization of the clinical field isolates of L. infantum,the phenotypic parameters have been less studied. In this study isolates from human and canine leishmaniasis (Hum1 and Can1) obtained in Campinas, São Paulo state, Brazil were identified as L. infantum. The Hum1 and Can1 isolates exhibited typical promastigote growth pattern. Regarding morphological features Can1 isolate differed in cell size. The infectivity in vitro of both isolatesis lower compared to the reference strain of L. infantum. Moreover, the in vivo infectivity of the three parasites is similar in Balb/c mice. The Hum1 isolate is more sensitive to leishmanial drugs (amphotericin B, miltefosine and glucantime) than the Can1 isolate when inside human macrophages, but not when inside canine macrophages. These findings indicated that L. infantum isolates differs in some phenotypic characteristics. |
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| Keywords: | Visceral leishmaniasis Canine leishmaniasis Clinical isolates Mice Macrophage |
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