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Serum amyloid A (SAA) mRNA expression in chicken and quails in response to bacterial stress

Institution:	1. Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran;2. Department of Food Hygiene and Aquatic, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran;3. Master Student of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran;1. Food Safety and Enteric Pathogens Research Unit, U.S. Department of Agriculture, Agricultural Research Services, National Animal Disease Center, Ames, IA, USA;2. Department of Animal Sciences, Purdue University, West Lafayette, IN, USA;3. Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada;4. Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA, USA;1. Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Selangor, Malaysia;2. Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, Malaysia;3. Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Selangor, Malaysia;4. Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Selangor, Malaysia;5. Viral Oncogenesis Group, The Pirbright Institute, Pirbright, Woking, UK;6. The Jenner Institute, The Centre for Cellular and Molecular Physiology, Roosevelt Drive, Oxford, United Kingdom

Abstract:	Monitoring of acute phase proteins such as serum amyloid A at gene expression level may provide quick information about immune status of the host and its susceptibility towards common infections. Present study was carried out to evaluate and compare the mRNA expression of SAA gene in Rhode Island Red chicken (RIR) and Japanese quails using real time PCR analysis in response to inactivated Salmonella gallinarum culture. The results showed that expression of SAA gene was approximately 17–33 folds higher in case of birds administered with bacterial culture when compared to un-inoculated controls and expression was higher and quicker in case of quails than RIR chicken. The SAA genes from chicken and quail were cloned and upon sequence analysis it was observed that deduced amino acid sequence of SAA from chicken and quails were having approximately seven percent variation which might have significance in function of this protein in these species.

Keywords:	Chicken Japanese quail serum amyloid A Expression Real time PCR Cloning
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