Molecular characterization of quinolone resistance mechanisms and extended-spectrum β-lactamase production in Escherichia coli isolated from dogs |
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| Institution: | 1. Produção Aquática, ICBAS – Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Porto, Portugal;2. Ciências Químicas e das Biomoléculas, Escola Superior de Tecnologia de Saúde do Porto, Instituto Politécnico do Porto, Porto, Portugal;3. Clínica Veterinária, ICBAS, Universidade do Porto, Porto, Portugal;4. CIIMAR – Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Porto, Portugal;5. CECA – Centro de Estudos de Ciência Animal, ICETA – Instituto de Ciências e Tecnologias Agrárias e Agro-Alimentares, Universidade do Porto, Porto, Portugal;6. CBQF – Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Porto, Portugal;1. Centro de Biotecnologia e Química Fina (CBQF), Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal;2. National Reference Laboratory of Antimicrobial Resistance and Healthcare Associated Infections, National Institute of Health Dr Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisbon, Portugal;3. Instituto Português de Oncologia Francisco Gentil, E.P.E, 4200-072 Porto, Portugal;4. Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal;1. Departments of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, AL, United States;2. Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL, United States;3. Department of Mathematics and Statistics, College of Science and Mathematics, Auburn University, AL, United States;1. College of Veterinary Medicine, Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University (SCAU), Guangzhou 510642, China;2. Laboratory of Clinical Microbiology, Institute of Veterinary Medicine, Guangdong Academy of Agriculture Sciences, Guangzhou 510640, China;1. Northwest A&F University, Shaanxi 712100, China;2. National Institutes for Food and Drug Control, Beijing 100050, China;3. Shanghai Jiaotong University, Shanghai 200240, China;4. Department of Nutrition and Food Science, University of Maryland, College Park, MD 20742, USA;5. Joint Institute for Food Safety and Applied Nutrition, College Park, MD 20742, USA;1. Department of Veterinary Microbiology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, South Korea;2. Department of Infectious Diseases, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, South Korea;3. Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, South Korea;4. Department of Biotechnology, Inje University, 197 Injero, Gimhae 50834, South Korea |
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| Abstract: | The increasing prevalence of antimicrobial resistances is now a worldwide problem. Investigating the mechanisms by which pets harboring resistant strains may receive and/or transfer resistance determinants is essential to better understanding how owners and pets can interact safely. Here, we characterized the genetic determinants conferring resistance to β-lactams and quinolones in 38 multidrug-resistant Escherichia coli isolated from fecal samples of dogs, through PCR and sequencing. The most frequent genotype included the β-lactamase groups TEM (n = 5), and both TEM + CTX-M-1 (n = 5). Within the CTX-M group, we identified the genes CTX-M-32, CTX-M-1, CTX-M-15, CTX-M-55/79, CTX-M-14 and CTX-M-2/44. Thirty isolates resistant to ciprofloxacin presented two mutations in the gyrA gene and one or two mutations in the parC gene. A mutation in gyrA (reported here for the first time), due to a transversion and transition (TCG → GTG) originating a substitution of a serine by a valine in position 83 was also detected. The plasmid-encoded quinolone resistance gene, qnrs1, was detected in three isolates. Dogs can be a reservoir of genetic determinants conferring antimicrobial resistance and thus may play an important role in the spread of antimicrobial resistance to humans and other co-habitant animals. |
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| Keywords: | Dogs Antimicrobial resistance β-Lactamases Quinolones |
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