Effects of aroA deleted E. coli vaccine on intestinal microbiota and mucosal immunity |
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| Institution: | 1. Imunova Análises Biológicas, Setor de Ciências Biológicas UFPR, Curitiba, PR, 81531-980, Brazil;2. Universidade Federal do Paraná, Setor de Ciências Biológicas UFPR, Curitiba, 81531-980, Brazil;3. Zoetis Indústria de Produtos Veterinários Ltda, São Paulo, SP, 04709-111, Brazil;1. Laboratorio de Brucelosis y Tuberculosis, Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia (FMVZ), Universidad Nacional Autónoma de México (UNAM), Ciudad de México, Mexico;2. Laboratorio de Microbiología Veterinaria, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas (ENCB), Instituto Politécnico Nacional (IPN), Ciudad de México 11340, Mexico;3. Laboratorio de Virología, Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia (FMVZ), Universidad Nacional Autónoma de México (UNAM), Ciudad de México, Mexico;4. Laboratorio de Bacteriología Médica, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas (ENCB), Instituto Politécnico Nacional (IPN), Ciudad de México 11340, Mexico;5. Unidad de Investigación en Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Ciudad de México 04510, Mexico;6. Área Académica de Nutrición y Toxicología Clínica, Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Pachuca Hidalgo, Mexico;7. Laboratorio de Inmunidad Innata, Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas (ENCB), Instituto Politécnico Nacional (IPN), Ciudad de México 11340, Mexico;8. Laboratorio de Inmunomicrobiología, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas (ENCB), Instituto Politécnico Nacional (IPN), Ciudad de México 11340, Mexico;1. Department of Medical Laboratory Techniques, Nasiriyah Technical Institute, Southern Technical University, Nasiriyah 64001, Iraq;2. Department of Biology, College of Education for Pure Sciences, University of Thi-Qar, Nasiriyah 64001, Iraq;1. Department of Clinical Sciences, Faculty of Veterinary Medicine, Sanandaj Branch Islamic Azad University, Sanandaj, Iran;2. Department of Microbiology, Faculty of Veterinary Medicine, Sanandaj Branch Islamic Azad University, Sanandaj, Iran;1. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran;2. Department of Medical Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran;3. Department of Geography, Ardabil Branch, Islamic Azad University, Ardabil, Iran;4. Genetic Engineering and Biotechnology Research Institute University of Sadat City Sadat, Egypt;5. Department of Parasitology and Mycology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran;6. Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran;7. Department of Medical Microbiology and Parasitology, College of Health Sciences, Bayero University, PMB 3011, Kano, Nigeria;8. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran;9. Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran;10. Department of Basic Sciences of Veterinary Medicine, Kazerun Branch, Islamic Azad University, Kazerun, Iran;11. Department of Parasitology, School of Allied Medical Sciences. Ilam University of Medical Sciences, Ilam, Iran |
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| Abstract: | E. coli infection of broilers can result in systemic diseases and productivity losses. Use of antimicrobials against this condition is common but other approaches, such as vaccination, are gaining ground. Anecdotal field reports indicate that intestinal health is improved unspecifically following E. coli live vaccination. We hypothesized that the intestine may be an important site for the functionality of the vaccine. Vaccine effects on the intestine were assessed. Spray vaccination induced marked alterations of the caecum microbiota of broilers within 3 days, and this effect gradually waned. However, T cell activation occurred in the spleen, but not in caecal tonsils, and anti-E. coli IgA was concentrated in the respiratory mucosae. Accordingly, IL-6 mRNA was produced in the lungs following immunization. Overall, these data are an initial indication that any vaccine-induced effects on the intestine are greatly associated with the microbiota. However, immunity conferred by vaccination is not primarily induced in gut-associated lymphoid tissues. |
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| Keywords: | Spray Vaccine IgA Microbiota Mucosal |
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