| O型口蹄疫病毒抗体化学发光免疫分析检测方法的建立 |
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| 引用本文： | 孙雨,宋晓晖,肖颖,王睿男,李秀梅,任雪建,李雪刚,魏巍,杨林,王传彬.O型口蹄疫病毒抗体化学发光免疫分析检测方法的建立[J].中国草食动物科学,2020(3):47-52. |
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| 作者姓名： | 孙雨 宋晓晖 肖颖 王睿男 李秀梅 任雪建 李雪刚 魏巍 杨林 王传彬 |
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| 作者单位： | 中国动物疫病预防控制中心;重庆市动物疫病预防控制中心;洛阳现代生物技术研究院有限公司;登封市畜牧业服务中心 |
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| 基金项目： | 国家重点计划研发项目(2016YFD0500901);现代农业人才支撑计划(2130106-024);跨境动物疫病防控机制建设(2130114-FK);农业技术实验示范与服务支持(净化)(2130106-039);现代农业产业技术体系建设北京市创新团队。 |
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| 摘 要： | 为建立动物血清中O型口蹄疫病毒抗体化学发光免疫分析检测方法,通过获得的O型口蹄疫病毒VP1融合蛋白与相关单克隆抗体,制备了O型口蹄疫病毒抗体竞争化学发光检测试剂盒。结果表明:该方法能够定量检测猪、牛、羊血清中的O型口蹄疫病毒VP1蛋白抗体,与其他偶蹄类动物病原无交叉反应,具有良好的重复性;对该方法的特异性指标、敏感性指标进行了验证,同国产O型口蹄疫抗体检测试剂盒进行了比较,阳性样品符合率92.62%,阴性样品符合率92.14%,总符合率92.45%;重复性试验组内与组间变异系数分别低于10%和15%。综上,该研究建立的O型口蹄疫病毒抗体化学发光免疫分析检测方法特异性高、敏感性强,操作简单、快速,具有较高的应用推广价值。
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| 关 键 词： | O型口蹄疫病毒 化学发光 免疫分析 检测方法 |
Establishment of Chemiluminescent Immunoassay for Detection of Type O FMDV Virus Antibody |
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| Sun Yu,Song Xiaohui,Xiao Ying,Wang Ruinan,Li Xiumei,Ren Xuejian,Li Xuegang,Wei Wei,Yang Lin,Wang Chuanbin.Establishment of Chemiluminescent Immunoassay for Detection of Type O FMDV Virus Antibody[J].China Herbivores,2020(3):47-52. |
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| Authors: | Sun Yu Song Xiaohui Xiao Ying Wang Ruinan Li Xiumei Ren Xuejian Li Xuegang Wei Wei Yang Lin Wang Chuanbin |
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| Institution: | (China Animal Disease Control Center,Beijing 102600,China;Chongqing Animal Disease Control Center,Chongqing 400000,China;Luoyang Modern Biotechnology Research Institute Co.,Ltd.,Luoyang,Henan 471000,China;Dengfeng Animal Disease Control Center,Dengfeng,Henan 452470,China) |
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| Abstract: | To establish chemiluminescence immunoassay method for Type O FMD virus antibody in animal serum,a competitive chemiluminescence assay kit for Type O FMD virus antibody was established by combining the obtained Type O FMD virus VP1 fusion protein with relevant monoclonal antibodies.The results showed that this method could specifically and quantitatively detect the antibody of Type O FMD virus in the serum of pigs,cattle and sheep.This method had high sensitivity and specificity,no cross-reaction to other related pathogens.Coefficient of variation within and between groups was less than 10%,and it had good repeatability.Compared with the domestic antibody detection kit for Type O FMD,the coincidence rate of positive samples was 92.62%,the coincidence rate of negative samples was 92.14%,and the total coincidence rate was 92.45%.The coefficient of variation within and between groups was less than 10%.The chemiluminescence immunoassay method for Type O FMD virus antibody established in this study is characterized by high specificity,high sensitivity,simple operation,high application and promotion value. |
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| Keywords: | Type O foot-and-mouth disease virus chemiluminescence immunoassay detection method |
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